{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T18:14:54Z","timestamp":1783188894798,"version":"3.54.6"},"reference-count":45,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,8,16]],"date-time":"2019-08-16T00:00:00Z","timestamp":1565913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81472728"],"award-info":[{"award-number":["81472728"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81672910"],"award-info":[{"award-number":["81672910"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>SALL4 is overexpressed in many cancers and is found to be involved in tumorigenesis and tumor progression. However, the function of SALL4 in cervical cancer remains unknown. Here, we showed that the expression of SALL4 was gradually increased from normal cervical tissue to high\u2010grade squamous intraepithelial lesions and then to squamous cervical carcinoma. SALL4 was upregulated or downregulated in cervical cancer cells by stably transfecting a SALL4\u2010expressing plasmid or a shRNA plasmid targeting SALL4, respectively. In vitro, cell growth curves and MTT (3\u2010(4,5\u2010dimethylthiazole\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide) assays showed that SALL4 promoted the cell proliferation of cervical cancer cells. In vivo, xenograft experiments verified that SALL4 enhanced the tumor formation of cervical cancer cells in female BALB\/c Nude mice. Cell cycle analysis by fluorescence\u2010activated cell sorting found that SALL4 accelerates cell cycle transition from the G<jats:sub>0<\/jats:sub>\/G<jats:sub>1<\/jats:sub> phase to the S phase. TOP\/FOP\u2010Flash reporter assay revealed that SALL4 significantly upregulates the activity of Wnt\/\u03b2\u2010catenin pathway. Western blotting showed that the expression levels of \u03b2\u2010catenin and important downstream genes, including c\u2010<jats:italic>Myc<\/jats:italic> and <jats:italic>cyclin D1<\/jats:italic>, were increased by SALL4 in cervical cancer cells. Furthermore, dual\u2010luciferase reporter and chromatin immunoprecipitation assays confirmed that SALL4 transcriptionally activated <jats:italic>CTNNB1<\/jats:italic> by physically interacting with its promoters. Taken together, The results of this study demonstrated that SALL4 may promote cell proliferation and tumor formation of cervical cancer cells by upregulating the activity of the Wnt\/\u03b2\u2010catenin signaling pathway by directly binding to the <jats:italic>CTNNB1<\/jats:italic> promoter and <jats:italic>trans\u2010<\/jats:italic>activating <jats:italic>CTNNB1<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/cas.14140","type":"journal-article","created":{"date-parts":[[2019,7,23]],"date-time":"2019-07-23T17:16:04Z","timestamp":1563902164000},"page":"2794-2805","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["SALL4 promotes the tumorigenicity of cervical cancer cells through activation of the Wnt\/\u03b2\u2010catenin pathway via <i>CTNNB1<\/i>"],"prefix":"10.1111","volume":"110","author":[{"given":"Mei","family":"Chen","sequence":"first","affiliation":[{"name":"The Department of Reproductive Medicine The First 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