{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T02:38:41Z","timestamp":1789094321164,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,8,13]],"date-time":"2019-08-13T00:00:00Z","timestamp":1565654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81472262"],"award-info":[{"award-number":["81472262"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81230057"],"award-info":[{"award-number":["81230057"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81730102"],"award-info":[{"award-number":["81730102"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>c\u2010Myb is a crucial transcription factor that participates in various biological functions; however, its role in colorectal cancer (<jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content>) remains poorly investigated. We first analyzed the expression and clinical significance of c\u2010Myb in a retrospective cohort enrolling 132 <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> patients. Then, the <jats:styled-content style=\"fixed-case\">CRISPR<\/jats:styled-content>\/Cas9 technique was used to establish c\u2010Myb gene <jats:styled-content style=\"fixed-case\">KO CRC<\/jats:styled-content> cell lines. Cellular functional assays in\u00a0vitro and in\u00a0vivo were used to evaluate the impact of c\u2010Myb <jats:styled-content style=\"fixed-case\">KO<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> cells. Finally, <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> sequencing was used to investigate the potential oncogenic mechanisms regulated by c\u2010Myb in <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> progression and related cellular validations were accordingly carried out. As a result, c\u2010Myb is significantly overexpressed in <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> tissues as compared with adjacent normal tissues. High expression of c\u2010Myb is positively correlated with lymph node metastasis and poor prognosis. Univariate analysis and multivariate analysis further identify c\u2010Myb as an independent unfavorable prognostic factor for <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> patients. c\u2010Myb <jats:styled-content style=\"fixed-case\">KO<\/jats:styled-content> inhibits the proliferation, apoptosis resistance, invasion, metastasis, colony formation and in\u00a0vivo tumorigenesis of <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> cells. Also, the mechanism investigation indicates that c\u2010Myb may promote <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> progression by regulating c\u2010fos. c\u2010fos overexpression can rescue the inhibitory effect of c\u2010Myb <jats:styled-content style=\"fixed-case\">KO<\/jats:styled-content> on the malignant characteristics of <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> cells. Finally, we find that c\u2010Myb <jats:styled-content style=\"fixed-case\">KO<\/jats:styled-content> inhibits the epithelial\u2010mesenchymal transition (<jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content>) molecular phenotype in <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> cells, whereas c\u2010fos overexpression can rescue this inhibitory effect. This study suggests that c\u2010Myb promotes the malignant progression of <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> through c\u2010fos\u2010induced <jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content> and has the potential to be a promising prognostic biomarker and therapeutic target.<\/jats:p>","DOI":"10.1111\/cas.14141","type":"journal-article","created":{"date-parts":[[2019,7,24]],"date-time":"2019-07-24T01:53:39Z","timestamp":1563933219000},"page":"3183-3196","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["c\u2010Myb promotes growth and metastasis of colorectal cancer through c\u2010fos\u2010induced epithelial\u2010mesenchymal transition"],"prefix":"10.1111","volume":"110","author":[{"given":"Xiao","family":"Qu","sequence":"first","affiliation":[{"name":"Department of General Surgery Shanghai Tenth People's Hospital Affiliated to Tongji University Shanghai 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