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Therefore, EP4 may play an important role in cancer progression. However, little information is available regarding their function per\u00a0se, including migration and the cellular signaling pathway of EP4 in oral cancer. First, we found that mRNA and protein expression of EP4 was abundantly expressed in human\u2010derived tongue squamous cell carcinoma cell lines HSC\u20103 and OSC\u201019. The EP4 agonist (ONO\u2010AE1\u2010437) significantly promoted cell migration in HSC\u20103 cells. In contrast, knockdown of EP4 reduced cell migration. Furthermore, we confirmed that knockdown of EP4 suppressed metastasis of oral cancer cells in the lungs of mice in\u00a0vivo. Therefore, we focused on the mechanism of migration\/metastasis in EP4 signaling. Interestingly, EP4 agonist significantly induced intracellular Ca<jats:sup>2+<\/jats:sup> elevation not in only oral cancer cells but also in other cells, including normal cells. Furthermore, we found that EP4 activated PI3K and induced Ca<jats:sup>2+<\/jats:sup> influx through Orai1 without activation of store depletion and stromal interaction molecule 1 (STIM1). Immunoprecipitation showed that EP4 formed complexes with Orai1 and TRPC1, but not with STIM. Moreover, the EP4 agonist ONO\u2010AE1\u2010437 phosphorylated ERK and activated MMP\u20102 and MMP\u20109. Knockdown of Orai1 negated EP4 agonist\u2010induced ERK phosphorylation. Taken together, our data suggested that EP4 activated PI3K and then induced Ca<jats:sup>2+<\/jats:sup> influx from the extracellular space through Orai1, resulting in ERK phosphorylation and promoting cell migration. 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