{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T23:05:56Z","timestamp":1782255956933,"version":"3.54.5"},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,12,20]],"date-time":"2019-12-20T00:00:00Z","timestamp":1576800000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007951","name":"Tokyo Medical and Dental University","doi-asserted-by":"publisher","award":["17K12005"],"award-info":[{"award-number":["17K12005"]}],"id":[{"id":"10.13039\/501100007951","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Claudins are the major component of tight junctions, which form a primary barrier to paracellular diffusion and maintain cell polarity in normal epithelia and endothelia. In cancer cells, claudins play additional roles besides serving as components of the tight junctions, and participate in anoikis or invasion. Among the claudin family proteins, claudin\u20101 has the most promising potential, both diagnostically and prognostically, in many types of cancers, including oral, gastric, liver, and colon cancers. However, conflicting results have been reported in relation to the degree of claudin\u20101 expression and the prognosis, suggesting that the expression level of claudin\u20101 alone is not sufficient to analyze the relationship between claudin\u20101 and cancer progression. As endocytic trafficking of claudin\u20101 has been reported in several epithelial cell types in\u00a0vitro, we aimed to determine whether intracellular localization of claudin\u20101 is the missing aspect between claudin\u20101 and cancer. We investigated the expression of claudin\u20101 in 83 tongue squamous cell carcinoma (TSCC) pathological specimens. Although the expression level of claudin\u20101 based on immunohistochemistry was not associated with TSCC progression, within the high claudin\u20101 expression group, the incidence of intracellular localization of claudin\u20101 was correlated with cervical lymph node metastasis. In an in\u00a0vitro experiment, claudin\u20101 was constitutively internalized in TSCC\u2010derived cells. Motility of TSCC\u2010derived cells was increased by deficiency of claudin\u20101, suggesting that the decrease in cell\u2010surface claudin\u20101 promoted the cell migration. Therefore, intracellular localization of claudin\u20101 at the invasion front may represent a promising diagnostic marker of TSCC.<\/jats:p>","DOI":"10.1111\/cas.14249","type":"journal-article","created":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T09:02:20Z","timestamp":1574758940000},"page":"700-712","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Intracellular claudin\u20101 at the invasive front of tongue squamous cell carcinoma is associated with lymph node metastasis"],"prefix":"10.1111","volume":"111","author":[{"given":"Daisuke","family":"Yamamoto","sequence":"first","affiliation":[{"name":"Department of Maxillofacial Surgery Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University (TMDU) Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kou","family":"Kayamori","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology Graduate 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