{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T02:25:45Z","timestamp":1790475945898,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,2,19]],"date-time":"2020-02-19T00:00:00Z","timestamp":1582070400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000646","name":"Japan Society for the Promotion of Science London","doi-asserted-by":"publisher","award":["Grant number: A16H069540"],"award-info":[{"award-number":["Grant number: A16H069540"]}],"id":[{"id":"10.13039\/501100000646","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although several studies have reported that microRNA (miR)\u201092b\u20103p is involved in various cellular processes related to carcinogenesis, its physiological role in clear cell renal cell carcinoma (ccRCC) remains unclear. To clarify the role of miR\u201092b\u20103p in ccRCC, we compared miR\u201092b\u20103p expression levels in ccRCC tissues and adjacent normal renal tissues. Significant upregulation of miR\u201092b\u20103p was observed in ccRCC tissues. Overexpression of miR\u201092b\u20103p using a miRNA mimic promoted proliferation, migration, and invasion activities of ACHN cells. Functional inhibition of miR\u201092b\u20103p by a hairpin miRNA inhibitor suppressed Caki\u20102 cell growth and invasion activities in vitro. Mechanistically, it was found that miR\u201092b\u20103p directly targeted the <jats:italic>TSC1<\/jats:italic> gene, a known upstream regulator of mTOR. Overexpression of miR\u201092b\u20103p decreased the protein expression of TSC1 and enhanced the downstream phosphorylation of p70S6 kinase, suggesting that the mTOR signaling pathway was activated by miR\u201092b\u20103p in RCC cells. Importantly, a multivariate Cox proportion hazard model, based on TNM staging and high levels of miR\u201092b\u20103p, revealed that miR\u201092b\u20103p expression (high vs. low hazard ratio, 2.86; 95% confidence interval, 1.20\u20106.83; <jats:italic>P<\/jats:italic>\u00a0=\u00a0.018) was a significant prognostic factor for overall survival of ccRCC patients with surgical management. Taken together, miR\u201092b\u20103p was found to act as an oncomiR, promoting cell proliferation by downregulating <jats:italic>TSC1<\/jats:italic> in ccRCC.<\/jats:p>","DOI":"10.1111\/cas.14325","type":"journal-article","created":{"date-parts":[[2020,1,24]],"date-time":"2020-01-24T04:00:00Z","timestamp":1579838400000},"page":"1146-1155","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["MicroRNA\u201092b\u20103p is a prognostic oncomiR that targets <i>TSC1<\/i> in clear cell renal cell carcinoma"],"prefix":"10.1111","volume":"111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8445-6205","authenticated-orcid":false,"given":"Cong","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motohide","family":"Uemura","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Suita Japan"},{"name":"Department of Urological Immuno\u2010Oncology Osaka University Graduate School of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eisuke","family":"Tomiyama","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Matsushita","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoko","family":"Koh","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kosuke","family":"Nakano","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4701-5635","authenticated-orcid":false,"given":"Yujiro","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Ishizuya","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kentaro","family":"Jingushi","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Physiology Osaka University Graduate School of Pharmaceutical Sciences Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8681-1407","authenticated-orcid":false,"given":"Taigo","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Suita Japan"},{"name":"Department of Urological Immuno\u2010Oncology Osaka University Graduate School of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Hatano","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9369-4264","authenticated-orcid":false,"given":"Atsunari","family":"Kawashima","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Ujike","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Nagahara","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazutoshi","family":"Fujita","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryoichi","family":"Imamura","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazutake","family":"Tsujikawa","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Physiology Osaka University Graduate School of Pharmaceutical Sciences Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norio","family":"Nonomura","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,2,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2042.2007.01739.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S56370"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3838"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molonc.2012.09.006"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3932"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2015.3799"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-14-0300"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2017.4016"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.4064"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2017.4080"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2013.2900"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-017-0723-7"},{"key":"e_1_2_8_14_1","first-page":"345","article-title":"Staging manual","volume":"14","author":"Greene FL","year":"2003","journal-title":"Ann Oncol"},{"key":"e_1_2_8_15_1","volume-title":"International Histological Classification of Tumors No. 25: Histological Typing of Kidney Tumors","author":"Mostofi FK","year":"1981"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/biomet\/57.3.579"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/biomet\/52.1-2.203"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu087"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1104"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0370217"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2013.07.031"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.09.111"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.06.106"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-017-2485-1"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-11-0302"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2016.3746"},{"issue":"4","key":"e_1_2_8_27_1","first-page":"6003","article-title":"MicroRNA\u2010183 promotes the proliferation and metastasis of renal cell carcinoma through targeting Dickkopf\u2011related protein 3","volume":"3","author":"Zhang XL","year":"2018","journal-title":"Oncology Letters"}],"container-title":["Cancer Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcas.14325","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cas.14325","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cas.14325","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cas.14325","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T11:46:32Z","timestamp":1693914392000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cas.14325"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,19]]},"references-count":26,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,4]]}},"alternative-id":["10.1111\/cas.14325"],"URL":"https:\/\/doi.org\/10.1111\/cas.14325","archive":["Portico"],"relation":{},"ISSN":["1347-9032","1349-7006"],"issn-type":[{"value":"1347-9032","type":"print"},{"value":"1349-7006","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,19]]},"assertion":[{"value":"2019-10-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}