{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T03:33:48Z","timestamp":1789788828880,"version":"4.0.1"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,2,29]],"date-time":"2020-02-29T00:00:00Z","timestamp":1582934400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20181129"],"award-info":[{"award-number":["BK20181129"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Estrogen\u2010related receptor\u2010\u03b1 (ERR\u03b1) is a nuclear receptor of transcription factor that binds to estrogen responsive elements and estrogen\u2010related responsive elements. Estrogen\u2010related receptor\u2010\u03b1 is involved in metabolic processes and implicated in the progression and growth of several human malignancies. However, the biologic role and clinical significance of ERR\u03b1 in gallbladder cancer (GBC) remains to be clarified. Here, we reported that ERR\u03b1 protein expression was notably higher in GBC tissues than in cholecystitis tissues, and that the aberrantly higher ERR\u03b1 expression was positively correlated with advanced TNM stage and indicated dismal prognosis of GBC (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a0.01). In GBC cell lines NOZ and OCUG, the targeted depletion of ERR\u03b1 retarded the growth and suppressed the migration and invasive capabilities of GBC cells, and inhibited epithelial\u2010mesenchymal transition by decreasing the expression of mesenchymal markers and elevating the expression of epithelial markers. Moreover, ERR\u03b1 knockdown inhibited tumor growth in nude mice and led to decreased expression levels of Nectin\u20104, p\u2010PI3K p85\u03b1, and p\u2010AKT. Overexpression of ERR\u03b1 in the GBC\u2010SD cell line showed exactly the opposite effect. The targeted inhibition of Nectin\u20104 antagonized GBC cell proliferation and invasion, which were induced by ERR\u03b1 upregulation. Moreover, Nectin\u20104 depletion inhibited the ERR\u03b1\u2010induced activation of the PI3K\/AKT pathway. Chromatin immunoprecipitation analysis and dual\u2010luciferase reporter gene assays showed that ERR\u03b1 enhanced the transcription of\n                    <jats:italic>Nectin\u20104<\/jats:italic>\n                    by binding to the promoter of\n                    <jats:italic>Nectin\u20104<\/jats:italic>\n                    . In conclusion, our data indicated that ERR\u03b1 could be a potential target for the genetic treatment of GBC.\n                  <\/jats:p>","DOI":"10.1111\/cas.14344","type":"journal-article","created":{"date-parts":[[2020,2,7]],"date-time":"2020-02-07T01:01:42Z","timestamp":1581037302000},"page":"1514-1527","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Estrogen\u2010related receptor\u2010\u03b1 promotes gallbladder cancer development by enhancing the transcription of Nectin\u20104"],"prefix":"10.1111","volume":"111","author":[{"given":"Lei","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Hepatopancreatobiliary Surgery The Affiliated Wuxi No. 2 People\u2019s Hospital of Nanjing Medical University Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MengMeng","family":"Yang","sequence":"additional","affiliation":[{"name":"Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology Jiangsu Institute of Parasitic Diseases Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingmei","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Hepatopancreatobiliary Surgery The Affiliated Wuxi No. 2 People\u2019s Hospital of Nanjing Medical University Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5883-9552","authenticated-orcid":false,"given":"Ziyi","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of General Surgery and Laboratory of General Surgery Xinhua Hospital, Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1700-4385","authenticated-orcid":false,"given":"Shilei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of General Surgery and Laboratory of General Surgery Xinhua Hospital, Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Hepatopancreatobiliary Surgery The Affiliated Wuxi No. 2 People\u2019s Hospital of Nanjing Medical University Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3442-5536","authenticated-orcid":false,"given":"Huihan","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Hepatopancreatobiliary Surgery The Affiliated Wuxi No. 2 People\u2019s Hospital of Nanjing Medical University Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,2,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2017.11.034"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canep.2018.04.011"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.13669"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-017-0107-3"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.2147\/CLEP.S155601"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v23.i22.3978"},{"key":"e_1_2_7_8_1","first-page":"99","article-title":"Gallbladder cancer: epidemiology and outcome","volume":"6","author":"Hundal R","year":"2014","journal-title":"Clin Epidemiol"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgt180"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2015.06.009"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-06-0227"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-0250"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/331091a0"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1677\/erc.1.01292"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-1251"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2009.02.010"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2011.08.023"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2006.10.012"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M103810200"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M203228200"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2010.07.003"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdw678"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2016.02.049"},{"key":"e_1_2_7_24_1","first-page":"1","article-title":"8th edition of the AJCC cancer staging manual: pancreas and hepatobiliary cancers","volume":"25","author":"Yun SC","year":"2018","journal-title":"Ann Surg Oncol"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-007-0558-5"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.584136"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.22363"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-11-3221"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3396"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc.2017.118"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2017.44"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-014-0276-y"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-15-1313"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-015-0144-7"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.00358"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-med-062913-051343"}],"container-title":["Cancer Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fcas.14344","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cas.14344","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cas.14344","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cas.14344","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T05:06:19Z","timestamp":1708923979000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cas.14344"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,29]]},"references-count":35,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,5]]}},"alternative-id":["10.1111\/cas.14344"],"URL":"https:\/\/doi.org\/10.1111\/cas.14344","archive":["Portico"],"relation":{},"ISSN":["1347-9032","1349-7006"],"issn-type":[{"value":"1347-9032","type":"print"},{"value":"1349-7006","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,29]]},"assertion":[{"value":"2019-10-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}