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The onset of MM is linked to exposure to asbestos, which generates reactive oxygen species (ROS). ROS are believed to be derived from the frustrated phagocytosis and the iron in asbestos. To explore the pathogenesis of MM, peritoneal MM was induced in rats by the repeated intraperitoneal injection of iron saccharate and nitrilotriacetate. In the present study, we used microarray techniques to screen the microRNA (miR) expression profiles of these MM. We observed that the histological subtype impacted the hierarchical clustering of miR expression profiles and determined that miR\u2010199\/214 is a distinctive feature of iron saccharate\u2010induced sarcomatoid mesothelioma (SM). Twist1, a transcriptional regulator of the epithelial\u2010mesenchymal transition, has been shown to activate miR\u2010199\/214 transcription; thus, the expression level of Twist1 was examined in iron\u2010induced and asbestos\u2010induced mesotheliomas in rats. Twist1 was exclusively expressed in iron saccharate\u2010induced SM but not in the epithelioid subtype. The Twist1\u2010miR\u2010199\/214 axis is activated in iron saccharate\u2010induced and asbestos\u2010induced SM. The expression levels of miR\u2010214 and Twist1 were correlated in an asbestos\u2010induced MM cell line, suggesting that the Twist1\u2010miR\u2010199\/214 axis is preserved. MeT5A, an immortalized human mesothelial cell line, was used for the functional analysis of miR. The overexpression of miR\u2010199\/214 promoted cellular proliferation, mobility and phosphorylation of Akt and ERK in MeT5A cells. These results indicate that miR\u2010199\/214 may affect the aggressive biological behavior of SM.<\/jats:p>","DOI":"10.1111\/cas.14405","type":"journal-article","created":{"date-parts":[[2020,4,5]],"date-time":"2020-04-05T05:38:32Z","timestamp":1586065112000},"page":"2016-2027","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Overexpression of miR\u2010199\/214 is a distinctive feature of iron\u2010induced and asbestos\u2010induced sarcomatoid mesothelioma in rats"],"prefix":"10.1111","volume":"111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1054-9403","authenticated-orcid":false,"given":"Yasumasa","family":"Okazaki","sequence":"first","affiliation":[{"name":"Department of Pathology and Biological Responses Nagoya University Graduate School of Medicine Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan 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