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Acquisition of CSC properties is highly associated with epigenetic alterations, including those mediated by microRNAs (miRNAs). We have previously established the breast cancer patient\u2010derived tumor xenograft (PDX) mouse model in which CSC marker CD44<jats:sup>+<\/jats:sup> cancer cells formed spontaneous microscopic metastases in the liver. In this PDX mouse, we found that the expression levels of 3 miRNAs (miR\u201025, miR\u201093, and miR\u2010106b) in the miR\u2010106b\u201025 cluster were much lower in the CD44<jats:sup>+<\/jats:sup> human cancer cells metastasized to the liver than those at the primary site. Constitutive overexpression of miR\u201093 suppressed invasive ability and 3D\u2010organoid formation capacity of breast cancer cells in vitro and significantly suppressed their metastatic ability to the liver in vivo. Wiskott\u2010Aldrich syndrome protein family member 3 (WASF3), a regulator of both cytoskeleton remodeling and CSC properties, was identified as a functional target of miR\u201093: overexpression of miR\u201093 reduced the protein level of WASF3 in breast cancer cells and WASF3 rescued the miR\u201093\u2010mediated suppression of breast cancer cell invasion. These findings suggest that miR\u201093 functions as a metastasis suppressor by suppressing both invasion ability and CSC properties in breast cancers.<\/jats:p>","DOI":"10.1111\/cas.14423","type":"journal-article","created":{"date-parts":[[2020,4,20]],"date-time":"2020-04-20T07:21:51Z","timestamp":1587367311000},"page":"2093-2103","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["MicroRNA\u201093 targets WASF3 and functions as a metastasis suppressor in breast cancer"],"prefix":"10.1111","volume":"111","author":[{"given":"Naoki","family":"Shibuya","sequence":"first","affiliation":[{"name":"Division of Molecular and Cellular Biology Kobe University Graduate School of Medicine Kobe Japan"},{"name":"Division of Gastrointestinal Surgery Kobe University Graduate School of Medicine Kobe 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