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Myostatin (MSTN)\/growth differentiation factor 8 (GDF\u20108), which is a member of the transforming growth factor\u2010\u03b2 family, is secreted in an inactivated form noncovalently bound to the prodomain, negatively regulating the skeletal muscle mass. Therefore, inhibition of MSTN signaling is expected to serve as a therapeutic target for intractable muscle wasting diseases. Here, we evaluated the inhibitory effect of peptide\u20102, an inhibitory core of mouse MSTN prodomain, on MSTN signaling. Peptide\u20102 selectively suppressed the MSTN signal, although it had no effect on the activin signal. In contrast, peptide\u20102 slightly inhibited the GDF\u201011 signaling pathway, which is strongly related to the MSTN signaling pathway. Furthermore, we found that the i.m. injection of peptide\u20102 to tumor\u2010implanted C57BL\/6 mice alleviated muscle wasting in cancer cachexia. Although peptide\u20102 was unable to improve the loss of heart weight and fat mass when cancer cachexia model mice were injected with it, peptide\u20102 increased the gastrocnemius muscle weight and muscle cross\u2010sectional area resulted in the enhanced grip strength in cancer cachexia mice. Consequently, the model mice treated with peptide\u20102 could survive longer than those that did not undergo this treatment. Our results suggest that peptide\u20102 might be a novel therapeutic candidate to suppress muscle wasting in cancer cachexia.<\/jats:p>","DOI":"10.1111\/cas.14520","type":"journal-article","created":{"date-parts":[[2020,6,10]],"date-time":"2020-06-10T07:39:16Z","timestamp":1591774756000},"page":"2954-2964","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Peptide\u20102 from mouse myostatin precursor protein alleviates muscle wasting in cancer\u2010associated cachexia"],"prefix":"10.1111","volume":"111","author":[{"given":"Chiharu","family":"Ojima","sequence":"first","affiliation":[{"name":"Laboratory of Cardiovascular Medicine Tokyo University of Pharmacy and Life Sciences Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri","family":"Noguchi","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Medicine Tokyo University of Pharmacy and Life Sciences Tokyo 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