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We conducted a multicenter prospective clinical sequencing study to verify the feasibility of a panel test for hematological tumors, including acute myeloid leukemia, acute lymphoblastic leukemia, multiple myeloma, and diffuse large B\u2010cell lymphoma. Out of 96 eligible patients, 79 patients (82%) showed potentially actionable findings, based on the clinical sequencing assays. We identified that genetic alterations with a strong clinical significance were found at a higher frequency in terms of diagnosis (n\u00a0=\u00a060; 63%) and prognosis (n\u00a0=\u00a061; 64%) than in terms of therapy (n\u00a0=\u00a08; 8%). Three patients who harbored a germline mutation in either\n                    <jats:italic>DDX41<\/jats:italic>\n                    (n\u00a0=\u00a02) or\n                    <jats:italic>BRCA2<\/jats:italic>\n                    (n\u00a0=\u00a01) were provided with genetic counseling. At 6 mo after sequencing, clinical actions based on the diagnostic (n\u00a0=\u00a05) or prognostic (n\u00a0=\u00a03) findings were reported, but no patients were enrolled in a clinical trial or received targeted therapies based on the sequencing results. These results suggest that panel testing for hematological malignancies would be feasible given the availability of useful diagnostic and prognostic information. This study is registered with the UMIN Clinical Trial Registry (UMIN000029879, multiple myeloma; UMIN000031343, adult acute myeloid leukemia; UMIN000033144, diffuse large B\u2010cell lymphoma; and UMIN000034243, childhood leukemia).\n                  <\/jats:p>","DOI":"10.1111\/cas.14552","type":"journal-article","created":{"date-parts":[[2020,7,3]],"date-time":"2020-07-03T11:27:54Z","timestamp":1593775674000},"page":"3367-3378","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Clinical utility of target capture\u2010based panel sequencing in hematological malignancies: A multicenter feasibility study"],"prefix":"10.1111","volume":"111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4686-0976","authenticated-orcid":false,"given":"Takahiko","family":"Yasuda","sequence":"first","affiliation":[{"name":"Clinical Research Center National Hospital Organization Nagoya Medical Center Nagoya 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