{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:07:06Z","timestamp":1785420426297,"version":"3.56.0"},"reference-count":32,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,9,15]],"date-time":"2020-09-15T00:00:00Z","timestamp":1600128000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Ibrutinib might improve the efficacy of anti\u2010CD19 chimeric antigen receptor (CD19 CAR) T\u2010cell therapy in chronic lymphocytic leukemia (CLL). We studied the possibility and mechanism of the synergistic effect of ibrutinib and CAR\u2010T cells in other types of lymphoma. In this study, we selected the CD19 CAR\u2010T cells of a patient with lymphoma who failed in his CD19 CAR\u2010T\u2010cell therapy and a dose of 8\u00a0mg\/kg\/d ibrutinib. Subcutaneous and tail vein tumorigenic mice were established with Raji cells. The differences in the synergistic effect between these 2 models were compared by bioluminescence imaging (BLI) monitoring and flow cytometry (FCM). The expression of the STAT\u20103 signaling pathway was assessed by western blot analysis. There was no synergistic effect of ibrutinib and CD19 CAR\u2010T cells in vitro. Programmed cell death\u2010ligand 1 (PD\u2010L1) was expressed in 0.23\u00a0\u00b1\u00a00.06% of Raji cells. In the subcutaneous tumorigenic model, the luciferase signal was reduced significantly in the group receiving ibrutinib combined with CD19 CAR\u2010T cells. Moreover, the proportion of CD19 CAR\u2010T cells was higher in the polytherapy group than in the CAR\u2010T\u2010cell monotherapy group. However, we did not get an analogous synergistic effect in the tail vein tumorigenic model. STAT\u20103 signaling pathway expression in the residual tumor cells did not differ between those with and those without ibrutinib, suggesting that the IL\u201010\/STAT\u20103\/PD\u2010L1 pathway was not involved in the synergistic effect. Therefore, some other mechanism might be a target for ibrutinib. Our results provide evidence for the use of ibrutinib in polytherapy for other types of B\u2010cell lymphoma.<\/jats:p>","DOI":"10.1111\/cas.14638","type":"journal-article","created":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T08:00:23Z","timestamp":1599033623000},"page":"4051-4060","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Synergistic effect of ibrutinib and CD19 CAR\u2010T cells on Raji cells in vivo and in vitro"],"prefix":"10.1111","volume":"111","author":[{"given":"Meijing","family":"Liu","sequence":"first","affiliation":[{"name":"The First Central Clinical College of Tianjin Medical University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuelin","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Developmental Biology and Molecular Medicine Fudan University Shanghai 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