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A recent clinical study of the dendritic cell vaccine pulsed with Tax peptides corresponding to CTL epitopes showed promising outcomes in ATL patients possessing limited human leukocyte antigen (HLA) alleles. In this study, we aimed to develop another immunotherapy to activate Tax\u2010specific CTL without HLA limitation by using patients\u2019 own HTLV\u20101\u2010infected cells as a vaccine. To examine the potential of HTLV\u20101\u2010infected T\u2010cells to activate CTL via antigen presenting cells, we established a unique co\u2013culture system. We demonstrated that mitomycin C\u2010treated HLA\u2010A2\u2010negative HTLV\u20101\u2010infected T\u2010cell lines or short\u2010term cultured peripheral blood mononuclear cells (PBMC) derived from ATL patients induced cross\u2013presentation of Tax antigen in co\u2013cultured HLA\u2010A2\u2010positive antigen presenting cells, resulting in activation of HLA\u2010A2\u2010restricted CD8<jats:sup>+<\/jats:sup> Tax\u2010specific CTL. This effect was not inhibited by a reverse transcriptase inhibitor. IL\u201012 production and CD86 expression were also induced in antigen presenting cells co\u2013cultured with HTLV\u20101\u2010infected cells at various levels, which were improved by pre\u2013treatment of the infected cells with histone deacetylase inhibitors. Furthermore, monocyte\u2010derived dendritic cells induced from PBMC of a chronic ATL patient produced IL\u201012 and expressed enhanced levels of CD86 when co\u2013cultured with autologous lymphocytes that had been isolated from the same PBMC and cultured for several days. These findings suggest that short\u2010term cultured autologous PBMC from ATL patients could potentially serve as a vaccine to evoke Tax\u2010specific CTL responses.<\/jats:p>","DOI":"10.1111\/cas.14800","type":"journal-article","created":{"date-parts":[[2021,1,10]],"date-time":"2021-01-10T12:51:40Z","timestamp":1610283100000},"page":"1161-1172","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Short\u2010term cultured autologous peripheral blood mononuclear cells as a potential immunogen to activate Tax\u2010specific CTL response in adult T\u2010cell leukemia patients"],"prefix":"10.1111","volume":"112","author":[{"given":"Miku","family":"Ishizawa","sequence":"first","affiliation":[{"name":"Deparment of Immunotherapeutics Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University Tokyo 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