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The neoplastic cells are derived from germinal center B cells that, in most cases, are infected by Epstein\u2010Barr virus (EBV), which may play a role in tumorigenesis. Given that EBV\u2010latent membrane protein 1 (LMP1) regulates autophagy in B cells, we explored the role of autophagy mediated by EBV or LMP1 in HL. We found that EBV\u2010LMP1 transfection in HL cells induced a modest increase in autophagy signals, attenuated starvation\u2010induced autophagic stress, and alleviated autophagy inhibition\u2010 or doxorubicin\u2010induced cell death. LMP1 knockdown leads to decreased autophagy LC3 signals. A xenograft mouse model further showed that EBV infection significantly increased expression of the autophagy marker LC3 in HL cells. Clinically, LC3 was expressed in 15% (19\/127) of HL samples, but was absent in all cases of nodular lymphocyte\u2010predominant and lymphocyte\u2010rich classic HL cases. Although expression of LC3 was not correlated with EBV status or clinical outcome, autophagic blockade effectively eradicated LMP1\u2010positive HL xenografts with better efficacy than LMP1\u2010negative HL xenografts. Collectively, these results suggest that EBV\u2010LMP1 enhances autophagy and promotes the viability of HL cells. Autophagic inhibition may be a potential therapeutic strategy for treating patients with HL, especially EBV\u2010positive cases.<\/jats:p>","DOI":"10.1111\/cas.14833","type":"journal-article","created":{"date-parts":[[2021,2,3]],"date-time":"2021-02-03T06:55:48Z","timestamp":1612335348000},"page":"1589-1602","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Epstein\u2010Barr virus latent membrane protein\u20101 upregulates autophagy and promotes viability in Hodgkin lymphoma: Implications for targeted therapy"],"prefix":"10.1111","volume":"112","author":[{"given":"Hui\u2010Chen","family":"Lin","sequence":"first","affiliation":[{"name":"Institute of Clinical Medicine College of Medicine National Cheng Kung University Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Chang","sequence":"additional","affiliation":[{"name":"National Institute of Infectious Diseases and Vaccinology National Health Research Institutes Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruo\u2010Yu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Bioindustry Sciences College of Bioscience and Biotechnology National Cheng Kung University Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang\u2010Yi","family":"Hung","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Bioindustry Sciences College of Bioscience and Biotechnology National Cheng Kung University Tainan Taiwan"},{"name":"PhD Program for Cancer Molecular Biology and Drug Discovery College of Medical Science and Technology Taipei Medical University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul Chih\u2010Hsueh","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology Veterans General Hospital\u2010Taipei Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010Ping","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Internal Medicine National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. Jeffrey","family":"Medeiros","sequence":"additional","affiliation":[{"name":"Department of Hematopathology The University of Texas M.D. Anderson Cancer Center Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Po\u2010Min","family":"Chiang","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine College of Medicine National Cheng Kung University Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9665-5236","authenticated-orcid":false,"given":"Kung\u2010Chao","family":"Chang","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine College of Medicine National Cheng Kung University Tainan Taiwan"},{"name":"Department of Pathology National Cheng Kung University Hospital College of Medicine National Cheng Kung University Tainan Taiwan"},{"name":"Department of Pathology Kaohsiung Medical University Hospital, College of Medicine, Kaohsiung Medical University 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