{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:29:54Z","timestamp":1787243394102,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008879","name":"Japanese Foundation for Prostate Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008879","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The prevalence of neuroendocrine prostate cancer (NEPC) arising from adenocarcinoma (AC) upon potent androgen receptor (AR) pathway inhibition is increasing. Deeper understanding of NEPC biology and development of novel therapeutic agents are needed. However, research is hindered by the paucity of research models, especially cell lines developed from NEPC patients. We established a novel NEPC cell line, KUCaP13, from tissue of a patient initially diagnosed with AC which later recurred as NEPC. The cell line has been maintained permanently in vitro under regular cell culture conditions and is amenable to gene engineering with lentivirus. KUCaP13 cells lack the expression of\n                    <jats:italic>AR<\/jats:italic>\n                    and overexpress NEPC\u2010associated genes, including\n                    <jats:italic>SOX2, EZH2, AURKA, PEG10, POU3F2, ENO2<\/jats:italic>\n                    , and\n                    <jats:italic>FOXA2<\/jats:italic>\n                    . Importantly, the cell line maintains the homozygous deletion of\n                    <jats:italic>CHD1<\/jats:italic>\n                    , which was confirmed in the primary AC of the index patient. Loss of heterozygosity of\n                    <jats:italic>TP53<\/jats:italic>\n                    and\n                    <jats:italic>PTEN<\/jats:italic>\n                    , and an allelic loss of\n                    <jats:italic>RB1<\/jats:italic>\n                    with a transcriptomic signature compatible with Rb pathway aberration were revealed. Knockdown of\n                    <jats:italic>PEG10<\/jats:italic>\n                    using shRNA significantly suppressed growth in vivo. Introduction of luciferase allowed serial monitoring of cells implanted orthotopically or in the renal subcapsule. Although H3K27me was reduced by EZH2 inhibition, reversion to AC was not observed. KUCaP13 is the first patient\u2010derived, treatment\u2010related NEPC cell line with triple loss of tumor suppressors critical for NEPC development through lineage plasticity. It could be valuable in research to deepen the understanding of NEPC.\n                  <\/jats:p>","DOI":"10.1111\/cas.14935","type":"journal-article","created":{"date-parts":[[2021,5,8]],"date-time":"2021-05-08T20:21:49Z","timestamp":1620505309000},"page":"2781-2791","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Establishment and characterization of a novel treatment\u2010related neuroendocrine prostate cancer cell line KUCaP13"],"prefix":"10.1111","volume":"112","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4378-096X","authenticated-orcid":false,"given":"Kosuke","family":"Okasho","sequence":"first","affiliation":[{"name":"Department of Urology Kyoto University Graduate School of Medicine Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kei","family":"Mizuno","sequence":"additional","affiliation":[{"name":"Department of Urology Kyoto University Graduate School of Medicine 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