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A lectin\u2010drug conjugate was generated by fusing rBC2LCN with a cytocidal toxin, and it showed a strong anticancer effect in in vitro and in vivo PDAC models. However, it is unclear which molecules are carrier proteins of rBC2LCN on PDAC cells. In this study, we identified a rBC2LCN\u2010positive glycoprotein expressed in PDAC. Tumor lysates of PDAC patient\u2010derived xenografts (PDXs) were coprecipitated with rBC2LCN lectin and analyzed by liquid chromatography\u2013mass spectrometry. A total of 343 proteins were initially identified. We used a web\u2010based database to select five glycoproteins and independently evaluated their expression in PDAC by immunohistochemistry (IHC). Among them, we focused on carcinoembryonic antigen 5 (CEA) as the most cancer\u2010specific carrier protein in PDAC, as it showed the most prominent difference in expression rate between PDAC cells (74%) and normal pancreatic duct cells (0%, <jats:italic>P<\/jats:italic>\u00a0&gt;\u00a0.0001). rBC2LCN lectin and CEA colocalization in PDAC samples was confirmed by double\u2010staining analysis. Furthermore, rBC2LCN\u2010precipitated fractions were blotted with an anti\u2010CEA polyclonal antibody (pAb), and CEA pAb\u2013precipitated fractions were blotted with rBC2LCN lectin. The results demonstrate that CEA is in fact a ligand of rBC2LCN lectin.<\/jats:p>","DOI":"10.1111\/cas.15023","type":"journal-article","created":{"date-parts":[[2021,6,11]],"date-time":"2021-06-11T17:05:24Z","timestamp":1623431124000},"page":"3722-3731","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Carcinoembryonic antigen as a specific glycoprotein ligand of rBC2LCN lectin on pancreatic ductal adenocarcinoma cells"],"prefix":"10.1111","volume":"112","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8843-9605","authenticated-orcid":false,"given":"Tomoaki","family":"Furuta","sequence":"first","affiliation":[{"name":"Faculty of Medicine Department of Surgery, Clinical Sciences University of Tsukuba Tsukuba Japan"},{"name":"Cellular and Molecular Biotechnology Research Institute National Institute of Advanced Industrial Science and Technology (AIST) Tsukuba 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Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5878-4459","authenticated-orcid":false,"given":"Sota","family":"Kimura","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Department of Surgery, Clinical Sciences University of Tsukuba Tsukuba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ko","family":"Kurimori","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Department of Surgery, Clinical Sciences University of Tsukuba Tsukuba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiro","family":"Miyazaki","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Department of Surgery, Clinical Sciences University of Tsukuba Tsukuba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Yu","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Department of Surgery, Clinical Sciences University 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