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Western blotting and fluorescence\u2010activated cell sorting analyses were used to determine CD70 expression in the ovarian cancer cell lines A2780 and SKOV3, and in the cisplatin\u2010resistant ovarian cancer cell lines A2780cisR and SKOV3cisR. CD70 expression after cisplatin exposure was determined in A2780 cells transfected with mock\u2010 or nuclear factor (NF)\u2010\u03baB\u2010p65\u2010small interfering RNA. We developed an ADC with an anti\u2010CD70 monoclonal antibody linked to monomethyl auristatin F and investigated its cytotoxic effect. We examined 63 ovarian cancer clinical samples; 43 (68.3%) of them expressed CD70. Among patients with advanced stage disease (<jats:italic>n<\/jats:italic>\u00a0=\u00a050), those who received neoadjuvant chemotherapy were more likely to exhibit high CD70 expression compared to those who did not (55.6% [15\/27] vs 17.4% [4\/23], <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01). CD70 expression was confirmed in A2780cisR, SKOV3, and SKOV3cisR cells. Notably, CD70 expression was induced after cisplatin treatment in A2780 mock cells but not in A2780\u2010NF\u2010\u03baB\u2010p65\u2010silenced cells. CD70\u2010ADC was cytotoxic to A2780cisR, SKOV3, and SKOV3cisR cells, with IC<jats:sub>50<\/jats:sub> values ranging from 0.104 to 0.341\u00a0nmol\/L. In A2780cisR and SKOV3cisR xenograft models, tumor growth in CD70\u2010ADC treated mice was significantly inhibited compared to that in the control\u2010ADC treated mice (A2780cisR: 32.0 vs 1639.0\u00a0mm<jats:sup>3<\/jats:sup>, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01; SKOV3cisR: 232.2 vs 584.9\u00a0mm<jats:sup>3<\/jats:sup>, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01). Platinum treatment induced CD70 expression in ovarian cancer cells. CD70\u2010ADC may have potential therapeutic implications in the treatment of CD70 expressing ovarian cancer.<\/jats:p>","DOI":"10.1111\/cas.15027","type":"journal-article","created":{"date-parts":[[2021,6,12]],"date-time":"2021-06-12T12:03:55Z","timestamp":1623499435000},"page":"3655-3668","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["CD70 antibody\u2010drug conjugate: A potential novel therapeutic agent for ovarian cancer"],"prefix":"10.1111","volume":"112","author":[{"given":"Mayu","family":"Shiomi","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Osaka University Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5725-9994","authenticated-orcid":false,"given":"Shinya","family":"Matsuzaki","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Osaka University Osaka 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