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In our previous reports, tissue\u2010clearing methods were used for the detection of metastatic tumors. Here, we showed that the cell cycles of primary and metastatic tumors were visualized by tissue\u2010clearing methods using a reporter system. First, we established cancer cell lines stably expressing fluorescent ubiquitination\u2010based cell cycle indicator (Fucci) reporter with widely used cancer cell lines A549 and 4T1. Fluorescence patterns of the Fucci reporter were investigated in various tumor inoculation models in mice. Interestingly, fluorescence patterns of the Fucci reporter of tumor colonies were different between various organs, and even among colonies in the same organs. The effects of antitumor drugs were also evaluated using these Fucci reporter cells. Of the three antitumor drugs studied, 5\u2010fluorouracil treatment on 4T1\u2010Fucci cells resulted in characteristic fluorescent patterns by the induction of G<jats:sub>2<\/jats:sub>\/M arrest both in vitro and in vivo. Thus, the combination of a tissue\u2010clearing method with the Fucci reporter is useful for analyzing the mechanisms of cancer metastasis and drug resistance.<\/jats:p>","DOI":"10.1111\/cas.15034","type":"journal-article","created":{"date-parts":[[2021,6,19]],"date-time":"2021-06-19T11:45:21Z","timestamp":1624103121000},"page":"3796-3809","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Visualization of the cancer cell cycle by tissue\u2010clearing technology using the Fucci reporter system"],"prefix":"10.1111","volume":"112","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7925-6636","authenticated-orcid":false,"given":"Kei","family":"Takahashi","sequence":"first","affiliation":[{"name":"Department of Molecular Pathology Graduate School of Medicine The University of Tokyo Tokyo 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