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However, the molecular mechanism by which specific ascites\u2010derived tumor cells survive and metastasize remains largely unknown. In this study, we elucidated cancer stem cell (CSC) properties of ascites\u2010derived MCSs, concomitant with enhanced malignancy, induced EMT, and low KLF9 (Kr\u00fcppel\u2010like factor 9) expression, compared with PTCs. KLF9 was also downregulated in OC cell line\u2010derived spheroids and the CD117<jats:sup>+<\/jats:sup>CD44<jats:sup>+<\/jats:sup> subpopulation in MCSs. Functional experiments demonstrated that KLF9 negatively modulated stem\u2010like properties in OC cells. Mechanistic studies revealed that KLF9 reduced the transcriptional expression of Notch1 by directly binding to the Notch1 promoter, thereby inhibiting the function of slug in a CSL\u2010dependent manner. Clinically, expression of KLF9 was associated with histological grade and loss of KLF9 predicts poor prognosis in OC.<\/jats:p>","DOI":"10.1111\/cas.15100","type":"journal-article","created":{"date-parts":[[2021,8,7]],"date-time":"2021-08-07T16:19:20Z","timestamp":1628353160000},"page":"4220-4233","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Loss of Kr\u00fcppel\u2010like factor 9 facilitates stemness in ovarian cancer ascites\u2010derived multicellular spheroids via Notch1\/slug signaling"],"prefix":"10.1111","volume":"112","author":[{"given":"Kun","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Qilu Hospital of Shandong University Ji'nan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shujie","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Qilu Hospital of Shandong University Ji'nan 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