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The \u03b32 chain (Lm\u2010\u03b32) is a frequently expressed monomer and its expression is closely associated with cancer progression. Laminin\u2010\u03b32 contains an epidermal growth factor (EGF)\u2010like domain in its domain III (DIII or LEb). Matrix metalloproteinases can cleave off the DIII region of Lm\u2010\u03b32 that retains the ligand activity for EGF receptor (EGFR). Herein, we show that a novel short form of Lm\u2010\u03b32 (Lm\u2010\u03b32F) containing DIII is generated without requiring MMPs and chromosomal translocation between LAMC2 on chromosome 1 and NR6A1 gene locus on chromosome 9 in human ovarian cancer SKOV3 cells. Laminin\u2010\u03b32F is expressed as a truncated form lacking domains I and II, which are essential for its association with Lm\u2010\u03b13 and \u2010\u03b23 chains of Lm\u2010332. Secreted Lm\u2010\u03b32F can act as an EGFR ligand activating the EGFR\/AKT pathways more effectively than does the Lm\u2010\u03b32 chain, which in turn promotes proliferation, survival, and motility of ovarian cancer cells. LAMC2\u2010NR6A1 translocation was detected using in situ hybridization, and fusion transcripts were expressed in ovarian cancer cell tissues. Overexpression and suppression of fusion transcripts significantly increased and decreased the tumorigenic growth of cells in mouse models, respectively.<\/jats:p><jats:p>To the best of our knowledge, this is the first report regarding a fusion gene of ECM showing that translocation of LAMC2 plays a crucial role in the malignant growth and progression of ovarian cancer cells and that the consequent product is a promising therapeutic target against ovarian cancers.<\/jats:p>","DOI":"10.1111\/cas.15149","type":"journal-article","created":{"date-parts":[[2021,10,24]],"date-time":"2021-10-24T14:13:58Z","timestamp":1635084838000},"page":"4957-4967","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Novel <i>LAMC2<\/i> fusion protein has tumor\u2010promoting properties in ovarian carcinoma"],"prefix":"10.1111","volume":"112","author":[{"given":"Hoshino","family":"Daisuke","sequence":"first","affiliation":[{"name":"Division of Cancer Cell Research Kanagawa Cancer Center Research Institute Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisamori","family":"Kato","sequence":"additional","affiliation":[{"name":"Division of Gynecology Kanagawa Cancer Center Hospital Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Fukumura","sequence":"additional","affiliation":[{"name":"Division of Gene Expression Mechanism Institute for Comprehensive Medical Science Fujita Health University Toyoake Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akila","family":"Mayeda","sequence":"additional","affiliation":[{"name":"Division of Gene Expression Mechanism Institute for Comprehensive Medical Science Fujita Health University Toyoake Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohei","family":"Miyagi","sequence":"additional","affiliation":[{"name":"Division of Molecular Pathology and Genetics Kanagawa Cancer Center Research Institute Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motoharu","family":"Seiki","sequence":"additional","affiliation":[{"name":"Division of Cancer Cell Research Institute of Medical Science University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4539-888X","authenticated-orcid":false,"given":"Naohiko","family":"Koshikawa","sequence":"additional","affiliation":[{"name":"Division of Cancer Cell Research Kanagawa Cancer Center Research Institute Yokohama Japan"},{"name":"Division of Cancer Cell Research Institute of Medical Science University of Tokyo Tokyo Japan"},{"name":"Department of Life Science and Technology Tokyo Institute of Technology Yokohama 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