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Skin cutaneous melanoma\u2014upregulated genes were screened via analysis of differentially expressed genes (GSE3189 and GSE46517), and metastasis\u2010related oncogenes were identified via weighted gene coexpression network analysis of the GSE46517 dataset. As confirmed by the Tumor Immune Estimation Resource, we found highly expressed centromere protein F (CENPF) in SKCM and its metastases. Immunostaining of human melanoma tissues demonstrated high CENPF expression. According to the Kaplan\u2010Meier survival curve log\u2010rank test, receiver\u2010operating characteristic curve, and univariate and multivariate analyses, the Cancer Genome Atlas (TCGA) database suggested CENPF be a typical independent predictor of SKCM. The CIBERSORT algorithm classified the types of the immune cells from GSE46517 and showed higher proportion of CD4+ memory\u2010activated T cells in metastatic melanoma. Single\u2010sample gene set enrichment analysis of TCGA data confirmed the correlation between CENPF and activated CD4+ T cells. Centromere protein F was positively correlated with tumor mutational burden and CD4+ memory T cell markers (interleukin [IL]\u201023A, CD28, and CD62L), negatively associated with memory T cell maintenance factors (IL\u20107 and IL\u201015) by correlation analysis. Moreover, immunofluorescence showed high coexpression of CENPF and IL23A, CD4 in melanoma. Upregulated CENPF might lead to premature depletion of CD4+ memory T cells and immunosuppression. Nomogram indicated CENPF clinical predictive value for 1\u2010, 3\u2010, 5\u2010, and 7\u2010year melanoma overall survival. Therefore, CENPF plays a vital role in the progression and metastasis of melanoma and can be an effective therapeutic target.<\/jats:p>","DOI":"10.1111\/cas.15303","type":"journal-article","created":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T12:07:10Z","timestamp":1645445230000},"page":"1220-1234","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["CENPF as an independent prognostic and metastasis biomarker corresponding to CD4+ memory T cells in cutaneous melanoma"],"prefix":"10.1111","volume":"113","author":[{"given":"Mengzhi","family":"Li","sequence":"first","affiliation":[{"name":"Department of Burns The First Affiliated Hospital of Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingling","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Burns The First Affiliated Hospital of Sun Yat\u2010sen University  Guangzhou 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