{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T19:32:33Z","timestamp":1790623953677,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T00:00:00Z","timestamp":1665964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The current success of mRNA vaccines against COVID\u201019 has highlighted the effectiveness of mRNA and DNA vaccinations. Recently, we demonstrated that a novel needle\u2010free pyro\u2010drive jet injector (PJI) effectively delivers plasmid DNA into the skin, resulting in protein expression higher than that achieved with a needle syringe. Here, we used ovalbumin (OVA) as a model antigen to investigate the potential of the PJI for vaccination against cancers. Intradermal injection of OVA\u2010expression plasmid DNA into mice using the PJI, but not a needle syringe, rapidly and greatly augmented OVA\u2010specific CD8<jats:sup>+<\/jats:sup> T\u2010cell expansion in lymph node cells. Increased mRNA expression of both interferon\u2010\u03b3 and interleukin\u20104 and an enhanced proliferative response of OVA\u2010specific CD8<jats:sup>+<\/jats:sup> T cells, with fewer CD4<jats:sup>+<\/jats:sup> T cells, were also observed. OVA\u2010specific in vivo killing of the target cells and OVA\u2010specific antibody production of both the IgG2a and IgG1 antibody subclasses were greatly augmented. Intradermal injection of OVA\u2010expression plasmid DNA using the PJI showed stronger prophylactic and therapeutic effects against the progression of transplantable OVA\u2010expressing E.G7\u2010OVA tumor cells. Even compared with the most frequently used adjuvants, complete Freund's adjuvant and aluminum hydroxide with OVA protein, intradermal injection of OVA\u2010expression plasmid DNA using the PJI showed a stronger CTL\u2010dependent prophylactic effect. These results suggest that the novel needle\u2010free PJI is a promising tool for DNA vaccination, inducing both a prophylactic and a therapeutic effect against cancers, because of prompt and strong generation of OVA\u2010specific CTLs and subsequently enhanced production of both the IgG2a and IgG1 antibody subclasses.<\/jats:p>","DOI":"10.1111\/cas.15542","type":"journal-article","created":{"date-parts":[[2022,8,24]],"date-time":"2022-08-24T13:13:40Z","timestamp":1661346820000},"page":"34-47","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Induction of potent antitumor immunity by intradermal <scp>DNA<\/scp> injection using a novel needle\u2010free pyro\u2010drive jet injector"],"prefix":"10.1111","volume":"114","author":[{"given":"Shinya","family":"Inoue","sequence":"first","affiliation":[{"name":"Department of Immunoregulation, Institute of Medical Science Tokyo Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izuru","family":"Mizoguchi","sequence":"additional","affiliation":[{"name":"Department of Immunoregulation, Institute of Medical Science Tokyo Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jukito","family":"Sonoda","sequence":"additional","affiliation":[{"name":"Department of Immunoregulation, Institute of Medical Science Tokyo Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eri","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Department of Immunoregulation, Institute of Medical Science Tokyo Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiro","family":"Katahira","sequence":"additional","affiliation":[{"name":"Department of Immunoregulation, Institute of Medical Science Tokyo Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideaki","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Department of Immunoregulation, Institute of Medical Science Tokyo Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aruma","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Immunoregulation, Institute of Medical Science Tokyo Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuma","family":"Furusaka","sequence":"additional","affiliation":[{"name":"Department of Immunoregulation, Institute of Medical Science Tokyo Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingli","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Immunoregulation, Institute of Medical Science Tokyo Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiko","family":"Yoneto","sequence":"additional","affiliation":[{"name":"Department of Immunoregulation, Institute of Medical Science Tokyo Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Sakaguchi","sequence":"additional","affiliation":[{"name":"Department of Device Application for Molecular Therapeutics, Graduate School of Medicine Osaka University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Terai","sequence":"additional","affiliation":[{"name":"Department of Device Application for Molecular Therapeutics, Graduate School of Medicine Osaka University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunihiko","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Department of Device Application for Molecular Therapeutics, Graduate School of Medicine Osaka University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2847-0341","authenticated-orcid":false,"given":"Takayuki","family":"Yoshimoto","sequence":"additional","affiliation":[{"name":"Department of Immunoregulation, Institute of Medical Science Tokyo Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,10,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.806641"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.2217\/fvl-2021-0170"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.2147\/RMHP.S309707"},{"key":"e_1_2_11_5_1","first-page":"159","article-title":"Intradermal vaccination to protect against yellow fever and influenza","volume":"351","author":"Roukens AH","year":"2012","journal-title":"Curr Top Microbiol Immunol"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.11.021"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2020.35693"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2017.08.006"},{"key":"e_1_2_11_9_1","unstructured":"YuC WalterM.Needleless injectors for the administration of vaccines: a review of clinical effectiveness.2020."},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xphs.2019.02.021"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1208\/s12249-019-1564-z"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2021.145664"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xphs.2020.11.020"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90434-D"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80447-1"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.1172"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-2084"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.70.6.849"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2013.00114"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/14760584.2019.1604231"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2009.10.005"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2020.12.008"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.68.6.793"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302827"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.29.1511"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.21651"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1218705110"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(20000620)68:6<697::AID-BIT14>3.0.CO;2-6"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevE.82.051602"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202004595"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2021.121200"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01643"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1159\/000232443"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1025092615584"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1208\/s12248-009-9143-y"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2016.00137"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2011.03.008"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.13823"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/jocd.14047"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-2815-6_22"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/mi12040435"},{"issue":"9","key":"e_1_2_11_43_1","first-page":"1537","article-title":"HER2\/neu DNA vaccination by intradermal gene delivery in a mouse tumor model: gene gun is superior to jet injector in inducing CTL responses and protective immunity","volume":"1","author":"Nguyen\u2010Hoai T","year":"2012","journal-title":"Onco Targets Ther"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(03)00275-5"}],"container-title":["Cancer Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cas.15542","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cas.15542","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cas.15542","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T13:54:05Z","timestamp":1692453245000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cas.15542"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,17]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1111\/cas.15542"],"URL":"https:\/\/doi.org\/10.1111\/cas.15542","archive":["Portico"],"relation":{},"ISSN":["1347-9032","1349-7006"],"issn-type":[{"value":"1347-9032","type":"print"},{"value":"1349-7006","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,17]]},"assertion":[{"value":"2022-03-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}