{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T13:52:05Z","timestamp":1788270725984,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T00:00:00Z","timestamp":1670457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP\u201019K09198"],"award-info":[{"award-number":["JP\u201019K09198"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The association between tumor microenvironment (TME) and cancer\u2010associated fibroblasts (CAFs) in intrahepatic cholangiocarcinoma (ICC) progression is poorly understood. This study aimed to reveal whether specific microRNAs (miRNAs) in extracellular vesicles (EVs) derived from CAFs were involved in ICC progression. Conditioned medium (CM) and EVs in the CM of CAFs and normal fibroblasts (NFs) derived from ICC specimens were used to investigate the effects on tumor cell lines. miRNA microarray assay was used to examine the miRNAs of EVs derived from CAFs and NFs in ICC, and the effects of miR\u2010493\u20105p on tumor cell lines were examined. Additionally, databases were used to identify miR\u2010493\u20105p targets, and the relationship between prognosis of ICC patients and cocaine\u2010 and amphetamine\u2010regulated transcript propeptide (CARTPT), one of the targets of miR\u2010493\u20105p, expression in ICC tissues was retrospectively analyzed. Compared with NF\u2010derived CM and EVs, CAF\u2010derived CM and EVs promoted cell lines in proliferation, scratch, migration, and invasion assays. miRNA microarray analysis revealed that miR\u2010493\u20105p was significantly increased in CAF\u2010derived EVs compared to NF\u2010derived EVs. Tumor cell lines transfected with miR\u2010493\u20105p were promoted in proliferation and scratch assays. Immunohistochemical staining was performed on 76 ICC specimens; both overall and recurrence\u2010free survival rates were significantly worse in the CARTPT\u2010negative group. Univariate and multivariate analyses showed that low CARTPT expression was an independent poor prognostic factor for overall and recurrence\u2010free survival. Overall, our data suggest that CAFs in the ICC TME suppress CARTPT in tumor cells and promote tumor cells via miR\u2010493\u20105p in EVs.<\/jats:p>","DOI":"10.1111\/cas.15644","type":"journal-article","created":{"date-parts":[[2022,11,12]],"date-time":"2022-11-12T08:48:12Z","timestamp":1668242892000},"page":"937-947","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Cancer\u2010associated fibroblasts promote tumor cell growth via <scp>miR<\/scp>\u2010493\u20105p in intrahepatic cholangiocarcinoma"],"prefix":"10.1111","volume":"114","author":[{"given":"Katsuya","family":"Toshida","sequence":"first","affiliation":[{"name":"Department of Surgery and Science, Graduate School of Medicine Kyushu University  Fukuoka 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