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Moreover, teratomas can develop from undifferentiated cells in induced pluripotent stem (iPS) cell transplantation therapy, seriously hampering the progress of regenerative medicine. Germinal center\u2010associated nuclear protein (GANP) is thought to be important to the biogenetic control of primordial germ cells and is among the genes susceptible to testicular germ cell tumors. Thus, we analyzed the expression of GANP in human testicular postpubertal\u2010type teratomas and established a novel mouse model to reveal the association between GANP and teratomagenesis. We analyzed 31 cases of human testicular postpubertal\u2010type teratomas and, in all cases, GANP was overexpressed. The aberrant expression was also detected in germ cell neoplasia in situ accompanied by the teratoma. GANP expression was particularly high in the epithelia of the epidermis, cutaneous appendages, and trachea\u2010like ciliated epithelium. To further clarify the association between GANP and teratomagenesis, we established a novel teratomagenesis mouse model (<jats:italic>CAG<\/jats:italic>\u2010<jats:italic>ganp<\/jats:italic><jats:sup>Tg<\/jats:sup> mice). In the GANP\u2212teratoma mice, GANP\u2010overexpressing teratomas were more frequent at the testes and the middle portion of the uterus than has been seen in the previously established mouse models. In conclusion, GANP is overexpressed in testicular postpubertal\u2010type teratomas and is an essential teratomagenic factor. We also found that <jats:italic>CAG<\/jats:italic>\u2010<jats:italic>ganp<\/jats:italic><jats:sup>Tg<\/jats:sup> mice are useful mouse models of teratomagenesis that mimics human midline teratomas and that teratomas may originate from the overexpression of GANP in primordial germ cells.<\/jats:p>","DOI":"10.1111\/cas.15685","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T17:29:54Z","timestamp":1670434194000},"page":"1729-1739","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Testicular teratomagenesis from primordial germ cells with overexpression of germinal center\u2010associated nuclear protein"],"prefix":"10.1111","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7210-9249","authenticated-orcid":false,"given":"Yasuhiro","family":"Sakai","sequence":"first","affiliation":[{"name":"Department of Joint Research Laboratory of Clinical Medicine Fujita Health University School of Medicine  Toyoake Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuya","family":"Yoshinaga","sequence":"additional","affiliation":[{"name":"Headquarters for Admission and Education Kumamoto University  Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayaka","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery Graduate School of Medical Sciences, Kumamoto University  Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andri","family":"Rezano","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Division of Cell Biology, Faculty of Medicine Universitas Padjadjaran  Sumedang Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuya","family":"Shiogama","sequence":"additional","affiliation":[{"name":"Department of Morphology and Pathological Diagnosis Fujita Health University School of Medical Sciences  Toyoake Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiaki","family":"Kawashima","sequence":"additional","affiliation":[{"name":"Department of Pathology Fujita Health University Bantane Hospital  Nagoya Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadashi","family":"Yoshizawa","sequence":"additional","affiliation":[{"name":"Department of Pathology and Bioscience Hirosaki University Graduate School of Medicine  Hirosaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiko","family":"Yoshizawa","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Kyoto University Hospital  Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0026-4079","authenticated-orcid":false,"given":"Shingo","family":"Hatakeyama","sequence":"additional","affiliation":[{"name":"Department of Urology Hirosaki University Graduate School 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