{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T22:36:44Z","timestamp":1792449404406,"version":"4.5.0"},"reference-count":58,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,4,3]],"date-time":"2023-04-03T00:00:00Z","timestamp":1680480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20K07704"],"award-info":[{"award-number":["20K07704"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Small extracellular vesicles (sEV) contain various microRNAs (miRNAs) and play crucial roles in the tumor metastatic process. Although miR\u201029b levels in peritoneal exosomes were markedly reduced in patients with peritoneal metastases (PM), their role has not been fully clarified. In this study, we asked whether the replacement of miR\u201029b can affect the development of PM in a murine model. UE6E7T\u201012, human bone marrow\u2010derived mesenchymal stem cells (BMSCs), were transfected with miR\u201029b\u2010integrating recombinant lentiviral vector and sEV were isolated from culture supernatants using ultracentrifugation. The sEV contained markedly increased amounts of miR\u201029b compared with negative controls. Treatment with transforming growth factor\u2010\u03b21 decreased the expression of E\u2010cadherin and calretinin with increased expression of vimentin and fibronectin on human omental tissue\u2010derived mesothelial cells (HPMCs). However, the effects were totally abrogated by adding miR\u201029b\u2010rich sEV. The sEV inhibited proliferation and migration of HPMCs by 15% (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.005,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096) and 70% (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.005,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096), respectively, and inhibited adhesion of NUGC\u20104 and MKN45 to HPMCs by 90% (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20095) and 77% (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20095), respectively. MicroRNA\u201029b\u2010rich murine sEV were similarly obtained using mouse BMSCs and examined for in vivo effects with a syngeneic murine model using YTN16P, a highly metastatic clone of gastric cancer cell. Intraperitoneal (IP) transfer of the sEV every 3\u2009days markedly reduced the number of PM from YTN16P in the mesentery (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096) and the omentum (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096). Bone marrow mesenchymal stem cell\u2010derived sEV are a useful carrier for IP administration of miR\u201029b, which can suppress the development of PM of gastric cancer.\n                  <\/jats:p>","DOI":"10.1111\/cas.15793","type":"journal-article","created":{"date-parts":[[2023,3,20]],"date-time":"2023-03-20T06:49:31Z","timestamp":1679294971000},"page":"2939-2950","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Intraperitoneal transfer of microRNA\u201029b\u2010containing small extracellular vesicles can suppress peritoneal metastases of gastric cancer"],"prefix":"10.1111","volume":"114","author":[{"given":"Yuki","family":"Kimura","sequence":"first","affiliation":[{"name":"Department of Surgery Jichi Medical University Hospital  Shimotsuke Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideyuki","family":"Ohzawa","sequence":"additional","affiliation":[{"name":"Department of Clinical 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