{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T19:39:36Z","timestamp":1784230776005,"version":"3.55.0"},"reference-count":32,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,6,11]],"date-time":"2023-06-11T00:00:00Z","timestamp":1686441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Classic Hodgkin lymphoma (cHL) is characterized by multinucleated cells called Reed\u2010Sternberg (RS) cells and genetic complexity. Although CD30 also characterizes cHL cells, its biological roles are not fully understood. In this report, we examined the link between CD30 and these characteristics of cHL cells. CD30 stimulation increased multinucleated cells resembling RS cells. We found chromatin bridges, a cause of mitotic errors, among the nuclei of multinucleated cells. CD30 stimulation induced DNA double\u2010strand breaks (DSBs) and chromosomal imbalances. RNA sequencing showed significant changes in the gene expression by CD30 stimulation. We found that CD30 stimulation increased intracellular reactive oxygen species (ROS), which induced DSBs and multinucleated cells with chromatin bridges. The PI3K pathway was responsible for CD30\u2010mediated generation of multinucleated cells by ROS. These results suggest that CD30 involves generation of RS cell\u2010like multinucleated cells and chromosomal instability through induction of DSBs by ROS, which subsequently induces chromatin bridges and mitotic error. The results link CD30 not only to the morphological features of cHL cells, but also to the genetic complexity, both of which are characteristic of cHL cells.<\/jats:p>","DOI":"10.1111\/cas.15874","type":"journal-article","created":{"date-parts":[[2023,6,12]],"date-time":"2023-06-12T01:06:16Z","timestamp":1686531976000},"page":"3433-3445","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<scp>CD30<\/scp> induces <scp>Reed\u2010Sternberg<\/scp> cell\u2010like morphology and chromosomal instability in classic Hodgkin lymphoma cell lines"],"prefix":"10.1111","volume":"114","author":[{"given":"Mariko","family":"Watanabe","sequence":"first","affiliation":[{"name":"Division of Hematology, Department of Laboratory Sciences, School of Allied Health Sciences Kitasato University  Sagamihara Japan"},{"name":"Department of Molecular Cell Therapy Kitasato University Graduate School of Medical Sciences  Sagamihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromi","family":"Hatsuse","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, School of Medicine Kitasato University  Sagamihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuaki","family":"Nagao","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics, School of Medicine Kitasato University  Sagamihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Nakashima","sequence":"additional","affiliation":[{"name":"Laboratory of Tumor Cell Biology, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaoru","family":"Uchimaru","sequence":"additional","affiliation":[{"name":"Laboratory of Tumor Cell Biology, Department of Computational Biology and Medical 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