{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T20:28:05Z","timestamp":1791836885777,"version":"4.3.4"},"reference-count":36,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,7,8]],"date-time":"2023-07-08T00:00:00Z","timestamp":1688774400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>CCL21\u2010Ser, a chemokine encoded by the <jats:italic>Ccl21a<\/jats:italic> gene, is constitutively expressed in the thymic epithelial cells and stromal cells of secondary lymphoid organs. It regulates immune cell migration and survival through its receptor CCR7. Herein, using CCL21\u2010Ser\u2010expressing melanoma cells and the <jats:italic>Ccl21a<\/jats:italic>\u2010deficient mice, we demonstrated the functional role of cancer cell\u2010derived CCL21\u2010Ser in melanoma growth in vivo. The B16\u2010F10 tumor growth was significantly decreased in <jats:italic>Ccl21a<\/jats:italic>\u2010deficient mice compared with that in wild\u2010type mice, indicating that host\u2010derived CCL21\u2010Ser contributes to melanoma proliferation in vivo. In <jats:italic>Ccl21a<\/jats:italic>\u2010deficient mice, tumor growth of melanoma cells expressing CCL21\u2010Ser was significantly enhanced, suggesting that CCL21\u2010Ser from melanoma cells promotes tumor growth in the absence of host\u2010derived CCL21\u2010Ser. The increase in tumor growth was associated with an increase in the CCR7<jats:sup>+<\/jats:sup> CD62L<jats:sup>+<\/jats:sup> T cell frequency in the tumor tissue but was inversely correlated with Treg frequency, suggesting that na\u00efve T cells primarily promote tumor growth. Adoptive transfer experiments demonstrated that na\u00efve T cells are preferentially recruited from the blood into tumors with melanoma cell\u2010derived CCL21\u2010Ser expression. These results suggest that CCL21\u2010Ser from melanoma cells promotes the infiltration of CCR7<jats:sup>+<\/jats:sup> na\u00efve T cells into the tumor tissues and creates a tumor microenvironment favorable for melanoma growth.<\/jats:p>","DOI":"10.1111\/cas.15902","type":"journal-article","created":{"date-parts":[[2023,7,8]],"date-time":"2023-07-08T05:46:19Z","timestamp":1688795179000},"page":"3509-3522","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>CCL21\u2010Ser<\/scp> expression in melanoma cells recruits <scp>CCR7<\/scp><sup>+<\/sup> na\u00efve T cells to tumor tissues and promotes tumor growth"],"prefix":"10.1111","volume":"114","author":[{"given":"Megumi","family":"Miyamoto","sequence":"first","affiliation":[{"name":"Department of Science, Graduate School of Science and Engineering Kindai University  Osaka 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