{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:49:25Z","timestamp":1747183765446,"version":"3.40.5"},"reference-count":36,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T00:00:00Z","timestamp":1693353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP19K09804"],"award-info":[{"award-number":["JP19K09804"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Indocyanine green (ICG) with near\u2010infrared (NIR) fluorescence imaging is used for lymphatic mapping. However, binding of ICG to blood proteins like serum albumin can shorten its retention time in sentinel lymph nodes (SLNs). Here, we investigated the efficacy and safety of a new fluorescence tracer comprising phytate and liposome (LP)\u2010encapsulated ICG. Coadministration of phytate with LP containing phosphatidic acid promotes chelation mediated by Ca<jats:sup>2+<\/jats:sup> in bodily fluids to enhance SLN retention. Uniformly sized LPs (100\u2009nm) encapsulating ICG under conditions that minimized fluorescence self\u2010quenching during storage were produced. We analyzed the behavior of the new tracer (ICG\u2010phytate\u2010LP) and control tracers (ICG and ICG\u2010LP) in the lymphatic flow of mice in terms of lymph node retention time. We also tested lymphatic flow and safety in pigs that have a more human\u2010like lymphatic system. LPs encapsulating stabilized ICG were successfully prepared. Mixing LP with phytate in the presence of Ca<jats:sup>2+<\/jats:sup> increased both the particle size and negative surface charge. In mice, ICG\u2010phytate\u2010LP had the best lymph node retention, with a fluorescence intensity ratio that increased over 6\u2009h and then decreased slowly over the next 24\u2009h. In pigs, administration of ICG and ICG\u2010phytate\u2010LP resulted in no death or weight loss. There were no obvious differences between blood test results for the ICG and ICG\u2010phytate\u2010LP groups, and the overall safety was good. ICG\u2010phytate\u2010LP may be a useful new tracer for gynecological cancers that require time for lymph node identification due to a retroperitoneal approach.<\/jats:p>","DOI":"10.1111\/cas.15927","type":"journal-article","created":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T00:29:07Z","timestamp":1693441747000},"page":"4216-4224","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Development of novel tracers for sentinel node identification in cervical cancer"],"prefix":"10.1111","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7031-6359","authenticated-orcid":false,"given":"Keisuke","family":"Kodama","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Graduate School of Medical Sciences, Kyushu University  Fukuoka 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