{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T19:04:24Z","timestamp":1791140664751,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,12,11]],"date-time":"2023-12-11T00:00:00Z","timestamp":1702252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21K07223"],"award-info":[{"award-number":["21K07223"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21K09353"],"award-info":[{"award-number":["21K09353"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Biomarkers that could detect the postoperative recurrence of upper tract urothelial carcinoma (UTUC) have not been established. In this prospective study, we aim to evaluate the utility of individualized circulating tumor DNA (ctDNA) monitoring using digital PCR (dPCR) as a tumor recurrence biomarker for UTUC in the perioperative period. Twenty\u2010three patients who underwent radical nephroureterectomy (RNU) were included. In each patient, whole exome sequencing by next\u2010generation sequencing and\n                    <jats:italic>TERT<\/jats:italic>\n                    promoter sequencing of tumor DNA were carried out. Case\u2010specific gene mutations were selected from sequencing analysis to examine ctDNA by dPCR analysis. We also prospectively collected plasma and urine ctDNA from each patient. The longitudinal variant allele frequencies of ctDNA during the perioperative period were plotted. Case\u2010specific gene mutations were detected in 22 cases (96%) from ctDNA in the preoperative samples. Frequently detected genes were\n                    <jats:italic>TERT<\/jats:italic>\n                    (39%),\n                    <jats:italic>FGFR3<\/jats:italic>\n                    (26%),\n                    <jats:italic>TP53<\/jats:italic>\n                    (22%), and\n                    <jats:italic>HRAS<\/jats:italic>\n                    (13%). In all cases, we obtained plasma and urine samples for 241 time points and undertook individualized ctDNA monitoring for 2\u2009years after RNU. Ten patients with intravesical recurrence had case\u2010specific ctDNA detected in urine at the time of recurrence. The mean lead time of urinary ctDNA in intravesical recurrence was 60\u2009days (range, 0\u2013202\u2009days). Two patients with distal metastasis had case\u2010specific ctDNA in plasma at the time of metastasis. In UTUC, tumor\u2010specific gene mutations can be monitored postoperatively as ctDNA in plasma and urine. Individualized ctDNA might be a minimally invasive biomarker for the early detection of postoperative recurrence.\n                  <\/jats:p>","DOI":"10.1111\/cas.16025","type":"journal-article","created":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T16:02:46Z","timestamp":1702396966000},"page":"529-539","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Postoperative recurrence detection using individualized circulating tumor\n                    <scp>DNA<\/scp>\n                    in upper tract urothelial carcinoma"],"prefix":"10.1111","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9498-9996","authenticated-orcid":false,"given":"Daichi","family":"Tamura","sequence":"first","affiliation":[{"name":"Department of Urology Iwate Medical University School of Medicine  Yahaba 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