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Thus, biological changes in the HB cell line HepG2 in response to induced ASCL2 expression were assessed. ASCL2 expression was induced in HepG2 cells using the Tet\u2010On 3G system, which includes doxycycline. Cell viability, proliferation activity, mobility, and stemness were evaluated using 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide, colony\u2010formation, migration, invasion, and sphere\u2010formation assays. Quantitative reverse\u2010transcription polymerase chain reaction was used to assess the expression of markers for proliferation (\n                    <jats:italic>CCND1<\/jats:italic>\n                    and\n                    <jats:italic>MYC<\/jats:italic>\n                    ), epithelial\u2010mesenchymal transition (EMT;\n                    <jats:italic>SNAI1<\/jats:italic>\n                    ,\n                    <jats:italic>TWIST1<\/jats:italic>\n                    , and\n                    <jats:italic>ZEB1<\/jats:italic>\n                    ), mesenchymal\u2010epithelial transition (\n                    <jats:italic>CDH1<\/jats:italic>\n                    ), and stemness (\n                    <jats:italic>KLF4<\/jats:italic>\n                    ,\n                    <jats:italic>POU5F1<\/jats:italic>\n                    , and\n                    <jats:italic>SOX9<\/jats:italic>\n                    ). Compared with the non\u2010induced HepG2 cells, cells with induced ASCL2 expression showed significant increases in viability, colony number, migration area (%), and sphere number on days 7, 14, 8, and 7, respectively, and invasion area (%) after 90\u2009h. Furthermore, induction of ASCL2 expression significantly upregulated\n                    <jats:italic>CCND1<\/jats:italic>\n                    ,\n                    <jats:italic>MYC<\/jats:italic>\n                    ,\n                    <jats:italic>POU5F1<\/jats:italic>\n                    ,\n                    <jats:italic>SOX9<\/jats:italic>\n                    , and\n                    <jats:italic>KLF4<\/jats:italic>\n                    expression on days 2, 2, 3, 3, and 5, respectively, and increased the ratios of\n                    <jats:italic>SNAI1<\/jats:italic>\n                    ,\n                    <jats:italic>TWIST1<\/jats:italic>\n                    , and\n                    <jats:italic>ZEB1<\/jats:italic>\n                    to\n                    <jats:italic>CDH1<\/jats:italic>\n                    on day 5. ASCL2 promoted the formation of malignant phenotypes in HepG2 cells, which may be correlated with the upregulation of the Wnt signaling pathway\u2010, EMT\u2010, and stemness\u2010related genes.\n                    <jats:italic>ASCL2<\/jats:italic>\n                    activation may therefore be involved in the progression of HB.\n                  <\/jats:p>","DOI":"10.1111\/cas.16051","type":"journal-article","created":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T23:54:18Z","timestamp":1704498858000},"page":"847-858","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Achaete\u2010scute family\n                    <scp>bHLH<\/scp>\n                    transcription factor 2 activation promotes hepatoblastoma progression"],"prefix":"10.1111","volume":"115","author":[{"given":"Yutaka","family":"Kato","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Graduate School of Biomedical and Health 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