{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T20:54:13Z","timestamp":1790974453541,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,2,27]],"date-time":"2024-02-27T00:00:00Z","timestamp":1708992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008238","name":"Hitachi","doi-asserted-by":"publisher","award":["2617Dm\u201008b"],"award-info":[{"award-number":["2617Dm\u201008b"]}],"id":[{"id":"10.13039\/100008238","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP22cm0106481"],"award-info":[{"award-number":["JP22cm0106481"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The urinary catecholamine metabolites, homovanillic acid (HVA) and vanillylmandelic acid (VMA), are used for the adjunctive diagnosis of neuroblastomas. We aimed to develop a scoring system for the diagnosis and pretreatment risk assessment of neuroblastoma, incorporating age and other urinary catecholamine metabolite combinations. Urine samples from 227 controls (227 samples) and 68 patients with neuroblastoma (228 samples) were evaluated. First, the catecholamine metabolites vanillactic acid (VLA) and 3\u2010methoxytyramine sulfate (MTS) were identified as urinary marker candidates through comprehensive analysis using liquid chromatography\u2013mass spectrometry. The concentrations of these marker candidates and conventional markers were then compared among controls, patients, and numerous risk groups to develop a scoring system. Participants were classified into four groups: control, low risk, intermediate risk, and high risk, and the proportional odds model was fitted using the L2\u2010penalized maximum likelihood method, incorporating age on a monthly scale for adjustment. This scoring model using the novel urine catecholamine metabolite combinations, VLA and MTS, had greater area under the curve values than the model using HVA and VMA for diagnosis (0.978 vs. 0.964), pretreatment risk assessment (low and intermediate risk vs. high risk: 0.866 vs. 0.724; low risk vs. intermediate and high risk: 0.871 vs. 0.680), and prognostic factors (<jats:italic>MYCN<\/jats:italic> status: 0.741 vs. 0.369, histology: 0.932 vs. 0.747). The new system also had greater accuracy in detecting missing high\u2010risk neuroblastomas, and in predicting the pretreatment risk at the time of screening. The new scoring system employing VLA and MTS has the potential to replace the conventional adjunctive diagnostic method using HVA and VMA.<\/jats:p>","DOI":"10.1111\/cas.16116","type":"journal-article","created":{"date-parts":[[2024,2,27]],"date-time":"2024-02-27T11:36:26Z","timestamp":1709033786000},"page":"1634-1645","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Scoring system for diagnosis and pretreatment risk assessment of neuroblastoma using urinary biomarker combinations"],"prefix":"10.1111","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5373-9069","authenticated-orcid":false,"given":"Hizuru","family":"Amano","sequence":"first","affiliation":[{"name":"Department of Rare\/Intractable Cancer Analysis Research Nagoya University Graduate School of Medicine  Nagoya Japan"},{"name":"Department of Pediatric Surgery Nagoya University Graduate School of Medicine  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroo","family":"Uchida","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Nagoya University Graduate School of Medicine  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuharu","family":"Harada","sequence":"additional","affiliation":[{"name":"Department of Health Data Science Tokyo Medical University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Narita","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Nagoya University Graduate School of Medicine  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigehisa","family":"Fumino","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Kyoto Prefectural University of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Yamada","sequence":"additional","affiliation":[{"name":"Children's Cancer Center National Center for Child Health and Development  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun","family":"Kumano","sequence":"additional","affiliation":[{"name":"Department of Rare\/Intractable Cancer Analysis Research Nagoya University Graduate School of Medicine  Nagoya Japan"},{"name":"Research &amp; Development Group, Hitachi, Ltd.  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayumi","family":"Abe","sequence":"additional","affiliation":[{"name":"Department of Rare\/Intractable Cancer Analysis Research Nagoya University Graduate School of Medicine  Nagoya Japan"},{"name":"Research &amp; Development Group, Hitachi, Ltd.  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Ishigaki","sequence":"additional","affiliation":[{"name":"Department of Rare\/Intractable Cancer Analysis Research Nagoya University Graduate School of Medicine  Nagoya Japan"},{"name":"Research &amp; Development Group, Hitachi, Ltd.  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Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shojiro","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Department of Complex Systems Science, Graduate School of Information Science Nagoya University  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaka","family":"Tomioka","sequence":"additional","affiliation":[{"name":"Department for the Promotion of Medical Device Innovation National Cancer Center Hospital East  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Muramatsu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Nagoya University Graduate School of Medicine  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuhiro","family":"Nishio","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Nagoya University Graduate School of Medicine  Nagoya 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