{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T11:07:28Z","timestamp":1785150448943,"version":"3.55.0"},"reference-count":43,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T00:00:00Z","timestamp":1717632000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The prognosis of patients with peripheral T\u2010cell lymphoma (PTCL) depends on bone marrow involvement (BMI). The bone marrow (BM) tumor microenvironment in PTCL remains unclear. We performed single\u2010cell RNA sequencing (scRNA\u2010seq) on 11 fresh BM samples from patients with BMI to reveal the associations of immune landscape and genetic variations with the prognosis of PTCL patients. Compared with PTCL not otherwise specified (NOS), angioimmunoblastic T\u2010cell lymphoma (AITL) had a higher number of T cells, lower number of lymphocytes, and greater inflammation. Immune heterogeneity in AITL is associated with prognosis. In particular, specific T\u2010cell receptor (TCR) T cells are enriched in patients with good response to anti\u2010CD30 therapy. We observed RhoA mutation\u2010associated neoantigens. Chidamide\u2010treated patients had a higher number of CD4+ regulatory cells and a better treatment response compared with other patients. In the nonresponder group, T\u2010cell enrichment progressed to secondary B\u2010cell enrichment and subsequently diffuse large B\u2010cell lymphoma. Moreover, AITL patients with lymphoma\u2010associated hemophagocytic syndrome had more T follicular helper (Tfh) cells with copy number variations in <jats:italic>CHR5<\/jats:italic>. To our knowledge, this study is the first to reveal the single\u2010cell landscape of BM microenvironment heterogeneity in PTCL patients with BMI. scRNA\u2010seq can be used to investigate the immune heterogeneity and genetic variations in AITL associated with prognosis.<\/jats:p>","DOI":"10.1111\/cas.16227","type":"journal-article","created":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T05:40:23Z","timestamp":1717738823000},"page":"2540-2552","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Single\u2010cell landscape reveals the immune heterogeneity of bone marrow involvement in peripheral T\u2010cell lymphoma"],"prefix":"10.1111","volume":"115","author":[{"given":"Jun","family":"Liu","sequence":"first","affiliation":[{"name":"Medical Research institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences) Southern Medical University  Guangzhou China"},{"name":"Department of Precision 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