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Recent progress uncovering the genetic characteristics associated with <jats:italic>MYD88<\/jats:italic>\/<jats:italic>CD79B<\/jats:italic> mutations has stimulated interest in the use of drugs targeting B\u2010cell receptor signaling, including Bruton's tyrosine kinase. However, difficulties in culturing ex\u00a0vivo IVLBCL cells has hampered research on the development of novel therapies. In the present study, we demonstrated the establishment of an ex\u00a0vivo culture system of IVLBCL cells obtained from patient\u2010derived xenograft (PDX) models. The spheroid culture enabled us to culture IVLBCL PDX cells for more than 10\u2009days and to explore the efficacy of drug treatments acting on these cells. We found that carfilzomib and ibrutinib were effective for treating IVLBCL in ex\u00a0vivo experiments and conducted in\u00a0vivo analyses to assess the efficacy of these drugs. Although the efficacy of carfilzomib was difficult to confirm due to its toxicity in our models, ibrutinib showed comparable efficacy to a standard combination of chemotherapy drugs. Together, our data provide a new culture method for IVLBCL PDX cells and a rationale for translating ibrutinib to clinical use in IVLBCL patients.<\/jats:p>","DOI":"10.1111\/cas.16310","type":"journal-article","created":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T01:42:57Z","timestamp":1729474977000},"page":"214-225","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Spheroid culture to select theoretical therapeutic drugs in intravascular large B\u2010cell lymphoma"],"prefix":"10.1111","volume":"116","author":[{"given":"Mika","family":"Takai","sequence":"first","affiliation":[{"name":"Department of Hematology and Oncology Nagoya University Graduate School of Medicine  Nagoya Aichi Japan"},{"name":"Department of Medical Innovations, Osaka Research Center for Drug Discovery Otsuka Pharmaceutical Co., Ltd.  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