{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:46:21Z","timestamp":1787013981584,"version":"build-2736575974"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T00:00:00Z","timestamp":1734652800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP20ck0106412h0003"],"award-info":[{"award-number":["JP20ck0106412h0003"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP23ck0106658h0003"],"award-info":[{"award-number":["JP23ck0106658h0003"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Melanoma\u2010associated antigen (MAGE)\u2010A4, a cancer testis antigen, presents a promising target for chimeric antigen receptor T cell therapy in refractory solid tumors, including breast cancer (BC). However, the lack of highly specific Abs against MAGE\u2010A4 is a major challenge for the development of MAGE\u2010A4\u2010targeted immunotherapies. This study aimed to validate the specificity of a novel MAGE\u2010A4 Ab (E701U) and examine MAGE\u2010A4 expression in clinical BC samples.\n                    <jats:italic>MAGE\u2010A1<\/jats:italic>\n                    ,\n                    <jats:italic>\u2010A2B<\/jats:italic>\n                    ,\n                    <jats:italic>\u2010A3<\/jats:italic>\n                    ,\n                    <jats:italic>\u2010A4<\/jats:italic>\n                    ,\n                    <jats:italic>\u2010A6<\/jats:italic>\n                    ,\n                    <jats:italic>\u2010A9<\/jats:italic>\n                    ,\n                    <jats:italic>\u2010A10,<\/jats:italic>\n                    and\n                    <jats:italic>\u2010A12<\/jats:italic>\n                    genes were transfected into HEK293 cells. MAGE\u2010A4 expression in each inserted cell block was evaluated using an E701U Ab. Subsequently, we evaluated MAGE\u2010A4 expression in 403 primary BC tissue samples by immunohistochemistry using E701U and analyzed the clinical impact of MAGE\u2010A4 in patients with early BC. The results showed that MAGE\u2010A4 expression was limited to cells transduced with the\n                    <jats:italic>MAGE\u2010A4<\/jats:italic>\n                    gene. MAGE\u2010A4 expression was observed in 5.7% of the BC samples. Positivity in triple\u2010negative BC was significantly higher than in the other subtypes. The 5\u2010year overall survival rate of patients with MAGE\u2010A4(+) was significantly worse than those with MAGE\u2010A4(\u2212) BC. Moreover, the 5\u2010year recurrence\u2010free survival (RFS) rate of patients with MAGE\u2010A4(+) BC was significantly lower than that of patients with MAGE\u2010A4(\u2212) BC. MAGE\u2010A4 expression was an independent prognostic factor for RFS. In conclusion, the E701U Ab showed reliable specificity for MAGE\u2010A4 expression among MAGE family genes. Patients with MAGE\u2010A4(+) BC have an unfavorable prognosis and represent potential candidates for MAGE\u2010A4\u2010specific immunotherapy.\n                  <\/jats:p>","DOI":"10.1111\/cas.16433","type":"journal-article","created":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T04:29:49Z","timestamp":1734668989000},"page":"744-752","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of\n                    <scp>MAGE<\/scp>\n                    \u2010\n                    <scp>A4<\/scp>\n                    expression in breast cancer and its impact on prognosis"],"prefix":"10.1111","volume":"116","author":[{"given":"Kaho","family":"Nakamura","sequence":"first","affiliation":[{"name":"Department of Breast Surgery Mie University Graduate School of Medicine  Tsu 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