{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T18:54:41Z","timestamp":1786215281004,"version":"build-2736575974"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T00:00:00Z","timestamp":1739404800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772727"],"award-info":[{"award-number":["81772727"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82172691"],"award-info":[{"award-number":["82172691"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Bladder cancer (BLCA) is one of the most common tumors of the urinary tract. The diagnosis of BLCA is mostly by invasive tests, which are damaging and unsuitable for early screening. Current non\u2010invasive diagnostic modalities are insufficient in sensitivity and specificity. Therefore, novel diagnostic markers are urgently needed to facilitate early detection of bladder cancer. tRNA\u2010derived small RNAs (tsRNAs) are considered to be novel and potentially biologically functional non\u2010coding RNAs (ncRNAs). tsRNAs have been used to help early diagnosis of a variety of tumors. However, whether tsRNAs in BLCA are altered or involved in BLCA progression or regulation remains unclear. Here, we identified a group of up\u2010regulated tsRNAs in BLCA by sequencing tsRNAs in the plasma of BLCA patients and normal controls and further screened two highly correlated tsRNAs with BLCA in the training set and validation set, which were named as tRF\u20101:28\u2010chrM.Ser\u2010TGA and tiRNA\u20101:34\u2010Glu\u2010CTC\u20101\u2010M2. ROC analyses of the expression profiles of these two tsRNAs by the validation set identified a high diagnostic value. We also found that circulating tRF\u20101:28\u2010chrM.Ser\u2010TGA and tiRNA\u20101:34\u2010Glu\u2010CTC\u20101\u2010M2 were specifically expressed and released by BLCA cells and were positively correlated with the degree of disease malignancy. In\u00a0vitro and in\u00a0vivo experiments revealed that the two tsRNAs exacerbated BLCA progression and played a role in promoting tumor lipid metabolism. Our study screened two plasma tsRNAs that could serve as valuable early screening and diagnostic biomarkers for BLCA and is also expected to provide potential novel molecular targets for the treatment of BLCA.<\/jats:p>","DOI":"10.1111\/cas.70003","type":"journal-article","created":{"date-parts":[[2025,2,14]],"date-time":"2025-02-14T05:20:30Z","timestamp":1739510430000},"page":"1255-1267","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Plasma <scp>tsRNA<\/scp> Signatures Serve as a Novel Biomarker for Bladder Cancer"],"prefix":"10.1111","volume":"116","author":[{"given":"Xinyan","family":"Xu","sequence":"first","affiliation":[{"name":"Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School Nanjing University  Nanjing China"},{"name":"State Key Laboratory of Pharmaceutical Biotechnology  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6148-8902","authenticated-orcid":false,"given":"Jinbang","family":"Chen","sequence":"additional","affiliation":[{"name":"Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School Nanjing University  Nanjing China"},{"name":"Nanjing Drum Tower Hospital Clinical College of Jiangsu University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Bai","sequence":"additional","affiliation":[{"name":"Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School Nanjing University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TianYao","family":"Liu","sequence":"additional","affiliation":[{"name":"Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School Nanjing University  Nanjing China"},{"name":"Tianjin Medical University Cancer Institute &amp; Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin Tianjin's Clinical Research Center for Cancer  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoubin","family":"Zhan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pharmaceutical Biotechnology  Nanjing Jiangsu China"},{"name":"Nanjing Drum Tower Hospital Clinical College of Jiangsu University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiazheng","family":"Li","sequence":"additional","affiliation":[{"name":"Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School Nanjing University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YuanChun","family":"Ma","sequence":"additional","affiliation":[{"name":"Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School Nanjing University  Nanjing 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