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We investigated the in\u00a0vitro effects of TP\u20100903 on a myelodysplastic syndrome (MDS)\u2010derived cell line (MDS\u2010L) and two myeloid leukemia cell lines. TP\u20100903 effectively inhibited cell proliferation and induced apoptosis in all three cell lines. In MDS\u2010L cells, the PI3K\/AKT and JAK\/STAT3 pathways were inhibited, suggesting that this may be partly due to decreased direct interactions with hepatocyte growth factor receptor, commonly known as MET. Regarding its effect on the cell cycle, TP\u20100903 was found to impact the DNA damage response and cell cycle\u2010related factors, particularly those centered around Aurora kinases. In MDS\u2010L cells, inhibition of Aurora A phosphorylation led to decreased levels of BORA, which in turn suppressed polo\u2010like kinase 1 activation. This suppression hindered mitosis initiation, resulting in cell cycle arrest at the G2\/M phase. Additionally, chromosomal misregulation caused by Aurora A inhibition appeared to impair cell division and contribute to cell death. Gene expression profiling of MDS\u2010L revealed changes in the ferroptosis\u2010related genes, including <jats:italic>HMOX1<\/jats:italic> and <jats:italic>transferrin<\/jats:italic>, along with elevated levels of reactive oxygen species and intracellular iron accumulation. These findings suggest the activation of an atypical ferroptosis pathway mediated through the TGF\u2010\u03b21\/SMAD3 signaling pathway. Overall, these data indicate that TP\u20100903 may offer a novel therapeutic strategy for the treatment of refractory hematological malignancies.<\/jats:p>","DOI":"10.1111\/cas.70151","type":"journal-article","created":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T03:49:31Z","timestamp":1753760971000},"page":"2831-2845","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>TP<\/scp>\u20100903 Suppresses Aurora A\u2013PLK1 Signaling to Inhibit Proliferation of a Myelodysplastic Syndrome\u2010Derived Cell Line"],"prefix":"10.1111","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2310-7965","authenticated-orcid":false,"given":"Tomoko","family":"Kimura\u2010Hyoda","sequence":"first","affiliation":[{"name":"Department of Health Sciences Graduate School of Medical Sciences, Kyushu University  Fukuoka 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