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However, the mechanism of how AR negatively regulates CCL2 expression is still unclear. As various microRNAs participate in prostate cancer development, we hypothesized that there are AR\u2010controlled miRs that regulate CCL2 production. Using LNCaP and C4\u20102B cells, miR\u2010124\u20103p.2 was found as a potential miR from the miRNA PCR array and public database. The expression of miR\u2010124\u20103p.2 was inhibited by knockdown AR, and the introduction of miR\u2010124\u20103p.2 mimic decreased CCL2 expression and suppressed cell migration. Dihydrotestosterone led to miR\u2010124\u20103p.2 upregulation and CCL2 downregulation. To examine whether miR\u2010124\u20103p.2 and CCL2 are involved in prostate cancer progression, their expression levels and clinical parameters were analyzed using prostate biopsy samples and whole blood RNAs obtained from the biopsied patients. Patients with low miR\u2010124\u20103p.2 and high CCL2 levels showed a shorter time to castration\u2010resistant prostate cancer development than those with high miR\u2010124\u20103p.2 and low CCL2 levels. Our study demonstrated that AR suppresses CCL2 expression via miR\u2010124\u20103p.2, and the miR\u2010124\u20103p.2\u2010CCL2 axis may be a novel therapeutic target and a useful blood prognostic biomarker for advanced prostate cancer.<\/jats:p>","DOI":"10.1111\/cas.70157","type":"journal-article","created":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T06:29:09Z","timestamp":1753252149000},"page":"2797-2807","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Androgen Receptor\u2010Controlled microRNA\u2010124\u20103p.2 Suppresses Prostate Cancer Progression via CCL2 Inhibition"],"prefix":"10.1111","volume":"116","author":[{"given":"Shuhei","family":"Aoyama","sequence":"first","affiliation":[{"name":"Department of Integrative Cancer Therapy and Urology Kanazawa University Graduate School of Medical Science  Kanazawa 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