{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T22:08:54Z","timestamp":1782511734430,"version":"3.54.5"},"reference-count":31,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Near\u2010infrared photoimmunotherapy (NIR\u2010PIT) is a tumor\u2010specific treatment using monoclonal antibody (mAb) photosensitizer conjugates, followed by near\u2010infrared light irradiation. This study aimed to identify the optimum target for treating esophagogastric junction (EGJ) adenocarcinoma and to evaluate the efficacy of NIR\u2010PIT using mAbs in preclinical models. Tumor samples from 46 consecutive patients who had undergone surgery without any prior treatment for EGJ adenocarcinoma were assessed for expression and homogeneity of epidermal growth factor receptor (EGFR), human epidermal growth factor receptor 2 (HER2), and epithelial cell adhesion molecule (EpCAM) through immunohistochemistry. Results showed positive rates of 22%, 13%, and 98% for EGFR, HER2, and EpCAM, respectively, with EpCAM also demonstrating the highest homogeneity (93%). Therefore, EpCAM was selected as the optimal target for NIR\u2010PIT. The fully human monoclonal antibody targeting EpCAM, adecatumumab, was conjugated with the photosensitizer IR700 at different dye\u2010antibody ratios (DAR2, DAR4, DAR7) and tested on OE19 cells and xenograft mouse models under near\u2010infrared light irradiation. NIR\u2010PIT with adecatumumab\u2010IR700 significantly reduced tumor size and improved prognoses compared to controls, with DAR2 showing the best balance of efficacy and minimal side effects. Notable EpCAM expression and homogeneity underpin EpCAM as a promising target for NIR\u2010PIT in EGJ adenocarcinoma. The fully human anti\u2010EpCAM antibody may be suitable for NIR\u2010PIT in EGJ adenocarcinoma.<\/jats:p>","DOI":"10.1111\/cas.70186","type":"journal-article","created":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:40:34Z","timestamp":1759336834000},"page":"3388-3402","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Near\u2010Infrared Photoimmunotherapy Targeting Esophagogastric Junction Adenocarcinoma With Fully Human Anti\u2010\n                    <scp>EpCAM<\/scp>\n                    Antibody"],"prefix":"10.1111","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5219-4706","authenticated-orcid":false,"given":"Maasa","family":"Sasabe","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Endoscopy National Cancer Center Hospital East  Kashiwa Chiba Japan"},{"name":"Course of Advanced Clinical Research of Cancer Juntendo University Graduate School of Medicine  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