{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T10:29:14Z","timestamp":1764584954203,"version":"3.46.0"},"reference-count":41,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T00:00:00Z","timestamp":1758412800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K08864","23K15832","24K02586"],"award-info":[{"award-number":["23K08864","23K15832","24K02586"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Choriocarcinoma is a rare gynecologic malignancy. MicroRNAs, which are noncoding RNAs approximately 22 nucleotides in length, are known to regulate gene expression and play important roles in various cancers; however, their functions in choriocarcinoma remain largely unknown. This study aimed to identify disease\u2010specific microRNAs involved in choriocarcinoma development. Eleven cases of choriocarcinoma and five cases of complete hydatidiform mole treated at our institution were analyzed. Total RNA was extracted from trophoblast cells in formalin\u2010fixed, paraffin\u2010embedded specimens using laser capture microdissection, and microRNA sequencing was performed. The analysis revealed that 87 microRNAs were significantly upregulated, whereas 28 were downregulated in choriocarcinoma compared to complete hydatidiform mole. Notably, 13 of the 28 downregulated microRNAs belonged to the chromosome 19 microRNA cluster. In\u00a0vitro experiments demonstrated that overexpression of miR\u2010517a\u20103p, a representative member of this cluster, significantly suppressed cell proliferation, migration, and invasion in JEG\u20103 and BeWo cell lines. Further transcriptome sequencing and computational analysis identified SRSF1 as a target gene of miR\u2010517a\u20103p, which was validated by dual\u2010luciferase reporter assays. Knockdown of SRSF1 also led to significant reductions in proliferation, migration, and invasion, supporting its functional relevance. Immunohistochemical analysis confirmed that SRSF1 protein was highly expressed in choriocarcinoma tissues compared to complete hydatidiform mole. These findings indicate that downregulation of the chromosome 19 microRNA cluster is a characteristic feature of choriocarcinoma and that miR\u2010517a\u20103p functions as a tumor suppressor by directly regulating SRSF1 expression.<\/jats:p>","DOI":"10.1111\/cas.70207","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T06:22:17Z","timestamp":1758522137000},"page":"3473-3486","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Downregulation of Chromosome 19\n                    <scp>miRNA<\/scp>\n                    Cluster and the Tumor\u2010Suppressive Role of\n                    <scp>miR<\/scp>\n                    \u2010517a\u20103p in Choriocarcinoma"],"prefix":"10.1111","volume":"116","author":[{"given":"Yuki","family":"Nishiko","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Nagoya University Graduate School of Medicine  Nagoya 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