{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T10:29:13Z","timestamp":1764584953349,"version":"3.46.0"},"reference-count":13,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T00:00:00Z","timestamp":1759449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22H0040"],"award-info":[{"award-number":["22H0040"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>CENP\u2010C, an essential component of the kinetochore, connects centromeric chromatin to the outer kinetochore, and thereby ensures accurate chromosome segregation. Although deletion of the Mis12\u2010binding domain (M12BD) of CENP\u2010C does not cause developmental disorders in mice, it promotes malignant tumor progression in the two\u2010stage DMBA\/TPA\u2010induced skin carcinogenesis model. In this study, we have demonstrated that M12BD deletion of CENP\u2010C enhances proliferation and then promotes abnormal differentiation in DMBA\/TPA\u2010induced carcinomas in mice. To elucidate the underlying molecular mechanisms, we performed RNA sequencing and found the dysregulated expression of keratinization\u2010related genes. Intriguingly, elevated chromosomal aneuploidy was detectable in mice with the M12BD deletion of CENP\u2010C. Among the aneuploidies, trisomies of chromosomes 6 and 10 took place at the highest frequency. These specific chromosomal gains were accompanied by upregulation of genes involved in immune and inflammatory responses. Together, our present findings strongly suggest that M12BD of CENP\u2010C plays a critical role in the regulation of epithelial differentiation during tumor development in mice.<\/jats:p>","DOI":"10.1111\/cas.70216","type":"journal-article","created":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T04:59:21Z","timestamp":1759553961000},"page":"3532-3539","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Deletion of the\n                    <scp>M<\/scp>\n                    is12\n                    <scp>C<\/scp>\n                    \u2010Binding Domain of\n                    <scp>CENP<\/scp>\n                    \u2010C Promotes Chromosomal Aneuploidy in Cutaneous Squamous Cell Carcinoma"],"prefix":"10.1111","volume":"116","author":[{"given":"Megumi","family":"Saito","sequence":"first","affiliation":[{"name":"Division of Experimental Animal Research Cancer Genome Center, Chiba Cancer Center Research Institute  Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Okumura","sequence":"additional","affiliation":[{"name":"Division of Experimental Animal Research Cancer Genome Center, Chiba Cancer Center Research Institute  Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yurika","family":"Tokunaga","sequence":"additional","affiliation":[{"name":"Division of Experimental Animal Research Cancer Genome Center, Chiba Cancer Center Research Institute  Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sora","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Division of Experimental Animal Research Cancer Genome Center, Chiba Cancer Center Research Institute  Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keisuke","family":"Otoyama","sequence":"additional","affiliation":[{"name":"Division of Experimental Animal Research Cancer Genome Center, Chiba Cancer Center Research Institute  Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinori","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Department of Applied Genomics Kazusa DNA Research Institute  Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masatoshi","family":"Hara","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Graduate School of Science The University of Tokyo  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masakazu","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Department of Cell Science, Institute of Biomedical Sciences, School of Medicine Fukushima Medical University  Fukushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihiko","family":"Fujimori","sequence":"additional","affiliation":[{"name":"Division of Embryology National Institute for Basic Biology  Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuo","family":"Fukagawa","sequence":"additional","affiliation":[{"name":"Graduate School of Frontier Biosciences The University of Osaka  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8861-2954","authenticated-orcid":false,"given":"Yuichi","family":"Wakabayashi","sequence":"additional","affiliation":[{"name":"Division of Experimental Animal Research Cancer Genome Center, Chiba Cancer Center Research Institute  Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,3]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature25432"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2024.102396"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.10.005"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.26508\/lsa.202402927"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.16246"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/sctm.20-0491"},{"key":"e_1_2_11_8_1","first-page":"17","article-title":"Skin Carcinogenesis Studies Using Mouse Models With Altered Polyamines","volume":"8","author":"Shannon L. 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