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However, only about 40% of patients respond to NAC, and tools to predict individual responses remain limited. The zebrafish patient\u2010derived xenograft (zPDX) model offers a rapid and cost\u2010effective platform for functional drug testing, but its application to MIBC has not yet been established. In this study, we developed a zPDX model optimized for evaluating CDDP sensitivity using clinical bladder cancer specimens. This model was validated through three steps: (1) evaluation of CDDP response in zPDXs derived from cell lines, (2) comparison of drug responses between mouse PDX (mPDX) and zPDX models, and (3) correlation of zPDX responses with clinical outcomes. The robustness of step 1 was demonstrated through complementary assays, including temperature optimization, in\u00a0vivo platinum quantification, fluorescent dye validation, Cap Analysis of Gene Expression (CAGE), and whole\u2010mount immunofluorescence. An image\u2010based platform for quantifying drug response by measuring fluorescent area was established and applied in steps 2 and 3. Step 2, using mPDXs, provided essential technical validation before applying the protocol to clinical samples. In step 3, patient\u2010derived tumors were transplanted into zebrafish, allowing successful prediction of CDDP efficacy. Despite the small patient cohort, this study provides fundamental evidence supporting zPDX as a clinically relevant and experimentally validated tool for functional assessment of CDDP sensitivity in bladder cancer.<\/jats:p>","DOI":"10.1111\/cas.70217","type":"journal-article","created":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:41:24Z","timestamp":1759920084000},"page":"3376-3387","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Zebrafish Xenograft Model for Predicting Cisplatin Efficacy in Muscle\u2010Invasive Bladder Cancer"],"prefix":"10.1111","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8361-0805","authenticated-orcid":false,"given":"Yusuke","family":"Sugino","sequence":"first","affiliation":[{"name":"Department of Nephro\u2010Urologic Surgery and Andrology Mie University Graduate School of Medicine  Tsu Japan"},{"name":"Center for Medical Zebrafish Research Mie University  Tsu 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