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Thus, it is likely that an inhibitor against this pro\u2010oncogenic pathway might be a promising anti\u2010cancer drug candidate. In the present study, we have synthesized a novel polyamide (WNT\u2010Chb) composed of\n                    <jats:italic>N<\/jats:italic>\n                    \u2010methylpyrrole,\n                    <jats:italic>N<\/jats:italic>\n                    \u2010methylimidazole, and DNA alkylator chlorambucil, and examined its anti\u2010cancer effect on colon cancer cells in\u00a0vitro and in\u00a0vivo. Based on our results, WNT\u2010Chb preferentially bound to a consensus TCF\/LEF\u2010responsive element (5\u2032\u2010CCTTTGA\u20103\u2032), and suppressed multiple Wnt\u2010target gene expression. Consistent with these results, WNT\u2010Chb attenuated in\u00a0vitro proliferation and in\u00a0vivo tumor growth of colon cancer cells with an aberrantly activated Wnt\/\u03b2\u2010catenin pathway. Together, these findings strongly suggest that the originally produced WNT\u2010Chb might be a novel anti\u2010cancer drug candidate against Wnt\/\u03b2\u2010catenin pathway\u2010dependent colon cancers.\n                  <\/jats:p>","DOI":"10.1111\/cas.70234","type":"journal-article","created":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T03:13:32Z","timestamp":1762398812000},"page":"203-215","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Targeting Wnt\/\u03b2\u2010Catenin Pathway by\n                    <scp>DNA<\/scp>\n                    Alkylating Pyrrole\u2010Imidazole Polyamide in Colon Cancer"],"prefix":"10.1111","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7508-5809","authenticated-orcid":false,"given":"Osamu","family":"Shimozato","sequence":"first","affiliation":[{"name":"Laboratory of Precision Tumor Model Systems Chiba Cancer Center Research Institute  Chiba 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