{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T02:08:14Z","timestamp":1791943694106,"version":"4.3.4"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100010795","name":"Chugai Pharmaceutical","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010795","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We investigated immunologic biomarkers that predict outcomes of patients with previously treated metastatic non\u2010small cell lung cancer who were enrolled in the J\u2010TAIL study, a prospective, observational study of atezolizumab monotherapy. Of 262 patients participating in the J\u2010TAIL exploratory study, peripheral blood mononuclear cells were obtained from 51 patients and analyzed by T\u2010cell fractionation analysis using Helios mass cytometry and serum proteomics analysis. Following treatment with atezolizumab, an increase in programmed cell death\u20101 (PD\u20101)\u2010expressing CD8 and CD4 T\u2010cell populations was observed. A more pronounced increase in PD\u20101 expression was seen in T cells from patients whose progression\u2010free survival (PFS) was 100\u2009days or longer compared with those with shorter PFS. The proximity extension assay, which is highly sensitive multiplex analysis technology that combines antibody\u2010based affinity assays with next\u2010generation sequencing, showed a significant increase in FOXO1, possibly in response to precursor\u2010exhausted T\u2010cell population activation. Immune\u2010related adverse events were associated with a high percentage of PD\u20101\u2010positive cells on effector memory CD8 T cells, which was thought to be accompanied by extremely high CD8 T\u2010cell activation. Further analysis distinguished poor prognosis populations with significant differences in CD62L\n                    <jats:sub>high<\/jats:sub>\n                    Th7R and CXCR3\n                    <jats:sup>+<\/jats:sup>\n                    component of Th7R (CXCR3\n                    <jats:sup>+<\/jats:sup>\n                    Th7R) within the population with PFS &lt;\u200950\u2009days. Patients with low Th7R or CXCR3\n                    <jats:sup>+<\/jats:sup>\n                    Th7R percentages prior to atezolizumab treatment had significantly poorer overall survival. These findings provide valuable insights regarding T\u2010cell kinetics and biomarkers in atezolizumab therapy and may offer promising directions for future research.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Trial Registration:<\/jats:bold>\n                    UMIN Clinical Trials Registry: UMIN000033133 and UMIN000035567;\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/clinicaltrials.gov\">ClinicalTrials.gov<\/jats:ext-link>\n                    : NCT03645330\n                  <\/jats:p>","DOI":"10.1111\/cas.70310","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T17:42:43Z","timestamp":1770054163000},"page":"904-916","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Analysis of Peripheral T Cell Profiling and Plasma Proteomics in Advanced\n                    <scp>NSCLC<\/scp>\n                    Patients Treated With Atezolizumab"],"prefix":"10.1111","volume":"117","author":[{"given":"Atsuto","family":"Mouri","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine, International Medical Center Saitama Medical University  Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Kagamu","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, International Medical Center Saitama Medical University  Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3098-2565","authenticated-orcid":false,"given":"Koji","family":"Tamada","sequence":"additional","affiliation":[{"name":"Department of Immunology Yamaguchi University Graduate School of Medicine  Yamaguchi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4969-4165","authenticated-orcid":false,"given":"Makoto","family":"Nishio","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medical Oncology The Cancer Institute Hospital of Japanese Foundation for Cancer Research  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5856-5512","authenticated-orcid":false,"given":"Hiroaki","family":"Akamatsu","sequence":"additional","affiliation":[{"name":"Internal Medicine III Wakayama Medical University  Wakayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7437-5054","authenticated-orcid":false,"given":"Yasushi","family":"Goto","sequence":"additional","affiliation":[{"name":"Department of Thoracic Oncology National Cancer Center Hospital  Tokyo 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