{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T16:09:00Z","timestamp":1777910940913,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T00:00:00Z","timestamp":1772409600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T00:00:00Z","timestamp":1772409600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003382","name":"Core Research for Evolutional Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003382","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Colorectal cancer (CRC) exhibits substantial molecular heterogeneity, yet detailed single\u2010cell transcriptomic data from Japanese patients remain limited. Here, we performed single\u2010cell RNA sequencing (scRNA\u2010seq) on tumor tissues and adjacent normal tissues from four Japanese CRC patients to characterize malignant cell populations. We identified a common cancer\u2010associated cell cluster observed across all four cases, characterized by high expression of the long noncoding RNA KCNQ1 opposite strand\/antisense transcript 1 (\n                    <jats:italic>KCNQ1OT1<\/jats:italic>\n                    ). Further analysis revealed a marked decrease in Ras Association Domain Family Member 6 (\n                    <jats:italic>RASSF6<\/jats:italic>\n                    ) expression within this cluster, despite clinical datasets reporting that elevated\n                    <jats:italic>RASSF6<\/jats:italic>\n                    expression correlates with poor overall survival in Western cohorts. Reanalysis of publicly available bulk RNA\u2010seq data from independent East Asian cohorts confirmed that\n                    <jats:italic>RASSF6<\/jats:italic>\n                    expression was consistently reduced in tumor tissues compared with adjacent normal colon tissues, while\n                    <jats:italic>KCNQ1OT1<\/jats:italic>\n                    expression showed no apparent differences across cohorts. Collectively, our single\u2010cell analysis delineates a recurrent cancer\u2010associated transcriptional state observed within this cohort and highlights distinct gene expression features that may be underappreciated by bulk or population\u2010averaged analyses, underscoring the value of single\u2010cell profiling for refining molecular interpretations of CRC.\n                  <\/jats:p>","DOI":"10.1111\/cas.70335","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T19:28:45Z","timestamp":1772998125000},"page":"1397-1411","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single\u2010Cell Analysis Identified a Recurrent Malignant\u2010Associated Transcriptional State in Colorectal Cancer"],"prefix":"10.1111","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0434-3054","authenticated-orcid":false,"given":"Sadahiro","family":"Iwabuchi","sequence":"first","affiliation":[{"name":"Department of Molecular Pathophysiology, Institute of Advanced Medicine Wakayama Medical University  Wakayama\u2010shi Wakayama Japan"},{"name":"Department of Bioinformatics and Genomics, Graduate School of Medical Sciences Kanazawa University  Kanazawa\u2010shi Ishikawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kentaro","family":"Sawada","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Gastrointestinal Oncology National Cancer Center Hospital East  Kashiwa\u2010shi, Chiba Japan"},{"name":"Department of Medical Oncology Kushiro Rosai Hospital  Kushiro\u2010shi Hokkaido Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6150-6545","authenticated-orcid":false,"given":"Motohiro","family":"Kojima","sequence":"additional","affiliation":[{"name":"Pathology Division, Research Center for Innovative Oncology National Cancer Center Hospital East  Kashiwa\u2010shi, Chiba Japan"},{"name":"Department of Surgical Pathology Kyoto Prefectural University of Medicine &amp; Graduate School of Medical Science  Kyoto\u2010shi Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miho","family":"Ozawa","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Gastrointestinal Oncology National Cancer Center Hospital East  Kashiwa\u2010shi, Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1407-6682","authenticated-orcid":false,"given":"Hiroya","family":"Taniguchi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Gastrointestinal Oncology National Cancer Center Hospital East  Kashiwa\u2010shi, Chiba Japan"},{"name":"Department of Clinical Oncology Aichi Cancer Center  Nagoya\u2010shi Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5041-2765","authenticated-orcid":false,"given":"Hideaki","family":"Bando","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Gastrointestinal Oncology National Cancer Center Hospital East  Kashiwa\u2010shi, Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazumi","family":"Abe","sequence":"additional","affiliation":[{"name":"Department of Computational Biology and Medical Sciences, Graduate School of Frontier Science The University of Tokyo  Kashiwa\u2010shi, Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuta","family":"Kuze","sequence":"additional","affiliation":[{"name":"Department of Computational Biology and Medical Sciences, Graduate School of Frontier Science The University of Tokyo  Kashiwa\u2010shi, Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyomi","family":"Imamura","sequence":"additional","affiliation":[{"name":"Department of Computational Biology and Medical Sciences, Graduate School of Frontier Science The University of Tokyo  Kashiwa\u2010shi, Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukie","family":"Kashima","sequence":"additional","affiliation":[{"name":"Department of Computational Biology and Medical Sciences, Graduate School of Frontier Science The University of Tokyo  Kashiwa\u2010shi, Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0608-3914","authenticated-orcid":false,"given":"Yoshihiko","family":"Hirohashi","sequence":"additional","affiliation":[{"name":"Department of Pathology Sapporo Medical University School of Medicine  Sapporo\u2010shi Hokkaido Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6808-5491","authenticated-orcid":false,"given":"Atsushi","family":"Tajima","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics and Genomics, Graduate School of Medical Sciences Kanazawa University  Kanazawa\u2010shi Ishikawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihiko","family":"Torigoe","sequence":"additional","affiliation":[{"name":"Department of Pathology Sapporo Medical University School of Medicine  Sapporo\u2010shi Hokkaido Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutaka","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Computational Biology and Medical Sciences, Graduate School of Frontier Science The University of Tokyo  Kashiwa\u2010shi, Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0489-4756","authenticated-orcid":false,"given":"Takayuki","family":"Yoshino","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Gastrointestinal Oncology National Cancer Center Hospital East  Kashiwa\u2010shi, Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinichi","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Department of Molecular Pathophysiology, Institute of Advanced Medicine Wakayama Medical University  Wakayama\u2010shi Wakayama 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