{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T18:39:11Z","timestamp":1790188751915,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T00:00:00Z","timestamp":1770681600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T00:00:00Z","timestamp":1770681600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100017691","name":"Guangxi Key Research and Development Program","doi-asserted-by":"publisher","award":["AB24010078"],"award-info":[{"award-number":["AB24010078"]}],"id":[{"id":"10.13039\/501100017691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017691","name":"Guangxi Key Research and Development Program","doi-asserted-by":"publisher","award":["AB25069454"],"award-info":[{"award-number":["AB25069454"]}],"id":[{"id":"10.13039\/501100017691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Epigenetic regulation plays a crucial role in the development of nasopharyngeal carcinoma (NPC). However, the epigenetic mechanisms underlying NPC recurrence and metastasis remain poorly understood. This study aimed to investigate and identify a novel molecular target with prognostic relevance in NPC. The methylation status of zinc finger protein 844 (\n                    <jats:italic>ZNF844<\/jats:italic>\n                    ) was assessed in NPC tissues and cell lines using bisulfite pyrosequencing.\n                    <jats:italic>ZNF844<\/jats:italic>\n                    expression levels in NPC cell lines and clinical specimens were analyzed by quantitative reverse transcription\u2010polymerase chain reaction (qRT\u2010PCR) and Western blotting. Stable NPC cell lines with\n                    <jats:italic>ZNF844<\/jats:italic>\n                    overexpression or knockdown were established to evaluate its biological functions both in\u00a0vitro and in\u00a0vivo. RNA sequencing was performed to identify downstream targets of\n                    <jats:italic>ZNF844<\/jats:italic>\n                    , followed by validation using qRT\u2010PCR and Western blotting. We found that\n                    <jats:italic>ZNF844<\/jats:italic>\n                    expression was significantly downregulated in NPC tissues and cell lines, correlating with hypermethylation of its promoter region. Treatment with the DNA methyltransferase inhibitor 5\u2010aza\u20102\u2032\u2010deoxycytidine (DAC) restored\n                    <jats:italic>ZNF844<\/jats:italic>\n                    mRNA expression.\n                    <jats:italic>ZNF844<\/jats:italic>\n                    overexpression suppressed NPC cell invasion and metastasis, whereas its silencing had the opposite effect. In xenograft models,\n                    <jats:italic>ZNF844<\/jats:italic>\n                    overexpression reduced metastatic burden.\n                    <jats:italic>ZNF844<\/jats:italic>\n                    inhibited the PI3K\u2010AKT signaling pathway, thereby suppressing NPC cell invasiveness. Clinically, high\n                    <jats:italic>ZNF844<\/jats:italic>\n                    expression was associated with improved overall survival and distant metastasis\u2010free survival.\n                    <jats:italic>ZNF844<\/jats:italic>\n                    inhibits the invasive and metastatic capabilities of nasopharyngeal carcinoma by modulating the PI3K\u2013AKT signaling pathway. These inhibitory effects can be partially or completely reversed by treatment with SC79. It may serve as a promising therapeutic target and prognostic biomarker for NPC.\n                  <\/jats:p>","DOI":"10.1111\/cas.70339","type":"journal-article","created":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T04:06:17Z","timestamp":1770782777000},"page":"943-957","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>ZNF844<\/scp>\n                    Suppresses Invasion and Metastasis in Nasopharyngeal Carcinoma by Inhibiting the\n                    <scp>PI3K<\/scp>\n                    \u2010\n                    <scp>AKT<\/scp>\n                    Signaling Pathway"],"prefix":"10.1111","volume":"117","author":[{"given":"Jinping","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Radiation Oncology Affiliated Hospital of Guilin Medical University  Guilin China"},{"name":"Key Laboratory of Tumor Functional Genomics and Precision Prevention and Treatment (Guilin Medical University) Education Department of Guangxi Zhuang Autonomous Region  Guilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cancan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Affiliated Hospital of Guilin Medical University  Guilin China"},{"name":"Key Laboratory of Tumor Functional Genomics and Precision Prevention and Treatment (Guilin Medical University) Education Department of Guangxi Zhuang Autonomous Region  Guilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Wuzhou Red Cross Hospital  Wuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shufang","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Affiliated Hospital of Guilin Medical University  Guilin China"},{"name":"Key Laboratory of Tumor Functional Genomics and Precision Prevention and Treatment (Guilin Medical University) Education Department of Guangxi Zhuang Autonomous Region  Guilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunyan","family":"Mo","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Affiliated Hospital of Guilin Medical University  Guilin China"},{"name":"Key Laboratory of Tumor Functional Genomics and Precision Prevention and Treatment (Guilin Medical University) Education Department of Guangxi Zhuang Autonomous Region  Guilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongjun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Affiliated Hospital of Guilin Medical University  Guilin China"},{"name":"Key Laboratory of Tumor Functional Genomics and Precision Prevention and Treatment (Guilin Medical University) Education Department of Guangxi Zhuang Autonomous Region  Guilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyun","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Affiliated Hospital of Guilin Medical University  Guilin China"},{"name":"Key Laboratory of Tumor Functional Genomics and Precision Prevention and Treatment (Guilin Medical University) Education Department of Guangxi Zhuang Autonomous Region  Guilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6534-8612","authenticated-orcid":false,"given":"Wei","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Affiliated Hospital of Guilin Medical University  Guilin China"},{"name":"Key Laboratory of Tumor Functional Genomics and Precision Prevention and Treatment (Guilin Medical University) Education Department of Guangxi Zhuang Autonomous Region  Guilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,10]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(19)30956-0"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-021-00524-x"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(15)70126-9"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(13)70181-5"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mds581"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-9525(87)90294-0"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-15-3278"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.6.2103"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra023075"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-016-0269-9"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cac2.12104"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-017-0621-2"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-4566"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-15-0260"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.21873\/cgp.20322"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2024.14728"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.14740\/wjon2547"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25031848"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abk2746"},{"issue":"10","key":"e_1_2_13_21_1","first-page":"6534","article-title":"LMO4 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