{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T14:23:01Z","timestamp":1792333381713,"version":"4.4.0"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T00:00:00Z","timestamp":1774828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T00:00:00Z","timestamp":1774828800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study evaluated the feasibility of urinary extracellular vesicles (EVs) as a liquid biopsy source for colorectal cancer (CRC) using two predefined cohorts: 97 patients for analytical validation and 63 patients for postoperative survival analysis. We compared urinary EVs, urinary cell\u2010free tumor DNA (ctDNA), plasma EVs, and plasma ctDNA across multiple molecular dimensions, and assessed the utility of urinary EV\u2010DNA for minimal residual disease (MRD) detection. Urinary EVs exhibited larger particle size (median 175.6\u2009nm) and markedly higher purity, demonstrated by a substantially broader proteomic profile (4674 vs. 476 proteins) and significantly higher expression of EV\u2010specific markers (CD63, CD81, CD9). DNA extracted from urinary EVs showed the highest concentration and best preservation of nucleic acid integrity, with the greatest Long\/Short fragment ratio, outperforming all plasma\u2010derived samples. Mutation detection sensitivity was comparable across sample types but was highest in plasma ctDNA (39.2%) and urinary EV\u2010DNA (34.0%), exceeding urinary ctDNA (21.6%). In the survival cohort, MRD positivity\u2014defined as detectable\n                    <jats:italic>RAS\/BRAF<\/jats:italic>\n                    mutations in urinary EV\u2010DNA 1\u2009month after curative surgery\u2014was strongly associated with inferior outcomes (HR for recurrence 5.25, 95% CI 1.74\u201315.80), and overall survival was significantly worse in MRD\u2010positive patients. These findings indicate that urinary EVs provide highly pure vesicles with superior DNA quality, making them a robust and completely non\u2010invasive source for molecular profiling. Urinary EV\u2010DNA\u2013based MRD assessment shows significant prognostic value and may serve as a practical tool for postoperative monitoring and risk stratification in colorectal cancer.\n                  <\/jats:p>","DOI":"10.1111\/cas.70368","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T08:29:36Z","timestamp":1774859376000},"page":"1667-1677","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Utility of Urinary Extracellular Vesicles for Colorectal Cancer: Comparison of DNA Quality and MRD Detection"],"prefix":"10.1111","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9466-2229","authenticated-orcid":false,"given":"Shintaro","family":"Kanaka","sequence":"first","affiliation":[{"name":"Department of Gastroenterological Surgery Nippon Medical School  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1436-7482","authenticated-orcid":false,"given":"Takeshi","family":"Yamada","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery Nippon Medical School  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumi","family":"Asai","sequence":"additional","affiliation":[{"name":"H.U. 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