{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T16:48:37Z","timestamp":1785775717457,"version":"3.56.0"},"reference-count":29,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T00:00:00Z","timestamp":1779062400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T00:00:00Z","timestamp":1779062400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100006606","name":"Natural Science Foundation of Tianjin Municipality","doi-asserted-by":"publisher","award":["23JCZXJC00040"],"award-info":[{"award-number":["23JCZXJC00040"]}],"id":[{"id":"10.13039\/501100006606","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006606","name":"Natural Science Foundation of Tianjin Municipality","doi-asserted-by":"publisher","award":["23JCZXJC00220"],"award-info":[{"award-number":["23JCZXJC00220"]}],"id":[{"id":"10.13039\/501100006606","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003787","name":"Natural Science Foundation of Hebei Province","doi-asserted-by":"publisher","award":["H2023206910"],"award-info":[{"award-number":["H2023206910"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC2508902"],"award-info":[{"award-number":["2023YFC2508902"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82270152"],"award-info":[{"award-number":["82270152"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2026,8]]},"abstract":"ABSTRACT<\/jats:title>\n \n Relapse after allogeneic hematopoietic stem cell transplantation (allo\u2010HSCT) remains the leading cause of treatment failure in acute myeloid leukemia (AML), yet the non\u2010coding RNA\u2010mediated regulatory mechanisms in relapse\u2010driving tumor clones are not fully understood. In this study, we performed transcriptomic profiling of CD34\n +<\/jats:sup>\n cells from the bone marrow of patients who had relapsed after allo\u2010HSCT and from those in sustained remission, and observed that long non\u2010coding RNAs (lncRNAs) exhibited the largest magnitude of differential expression relative to other RNA classes. To systematically elucidate their function, we integrated correlation analysis, neighborhood topology, and thermodynamic modeling to construct relapse\u2010associated competing endogenous RNA (ceRNA) networks. These networks highlighted multiple lncRNA\u2010miRNA\u2010mRNA axes potentially promoting leukemic progression. Functional interrogation using CRISPR\u2010based xenograft assays and a single\u2010cell CRISPR screening platform (Perturb\u2010seq\u2010like strategy) revealed that relapse\u2010associated lncRNAs regulate AML cell proliferation and survival. Among them, SNHG8 emerged as a representative regulator that promotes relapse through the SNHG8\u2010miR\u2010625\u2010ZC3H13\/15 signaling axis. SNHG8 knockdown markedly impaired AML cell growth and triggered apoptosis. Furthermore, treatment with hypomethylating agents (HMAs) such as azacitidine and decitabine differentially modulated lncRNA\/ceRNA expression signatures, thereby linking clinical interventions to transcriptomic regulation. Together, our findings identify lncRNA\u2010ceRNA networks in post\u2010transplant relapse of AML and identify the SNHG8 axis as a potential therapeutic vulnerability, providing a rationale for further investigation of lncRNA\u2010targeted strategies in this clinical setting.\n <\/jats:p>","DOI":"10.1111\/cas.70417","type":"journal-article","created":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T02:11:47Z","timestamp":1779156707000},"page":"2289-2304","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Transcriptomic and Functional Analyses Reveal\n lncRNA<\/scp>\n \u2010\n miRNA<\/scp>\n \u2010\n mRNA<\/scp>\n Axis in\n AML<\/scp>\n Relapse After Allo\u2010\n HSCT<\/scp>"],"prefix":"10.1111","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5708-4704","authenticated-orcid":false,"given":"Fei","family":"Zhao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Hematopoietic Stem Cell Transplantation Center, Institute of Hematology & Blood Diseases Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Tianjin P. R. China"},{"name":"Tianjin Institutes of Health Science Tianjin P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhao","family":"Wang","sequence":"additional","affiliation":[{"name":"Haihe Laboratory of Cell Ecosystem Tianjin Medical University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongye","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Hematopoietic Stem Cell Transplantation Center, Institute of Hematology & Blood Diseases Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Tianjin P. R. China"},{"name":"Tianjin Institutes of Health Science Tianjin P. R. 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