{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T10:30:34Z","timestamp":1793615434815,"version":"4.5.0"},"reference-count":42,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Tumor\u2010associated macrophages (TAMs) are key components of the immunosuppressive tumor microenvironment (TME) and represent attractive therapeutic targets to enhance antitumor immunity. However, systemic TAM\u2010depletion strategies targeting colony\u2010stimulating factor 1 receptor (CSF1R) are associated with the risk of off\u2010target toxicity due to the depletion of macrophages in healthy tissues. Here, we investigated the potential of near\u2010infrared photoimmunotherapy (NIR\u2010PIT) as a strategy for selective, photo\u2010induced elimination of TAMs. The Fab fragment of an anti\u2010CSF1R antibody was conjugated with the photosensitizer IRDye\u00a0700DX (IR700), and the therapeutic effects of the conjugate were evaluated in a murine cancer model. Upon NIR light irradiation, Fab\u2010IR700 significantly suppressed tumor growth and improved overall survival of the tumor\u2010bearing mice. Notably, repeated NIR\u2010PIT cycles enhanced therapeutic outcomes and induced durable anticancer effects, as indicated by the rejection of reimplanted cancer cells. Flow cytometry and histological analyses confirmed TAM depletion and significant alterations in the intra\u2010tumoral immune cell populations posttreatment. Overall, CSF1R\u2010targeted NIR\u2010PIT can effectively eliminate TAMs without systemic toxicity, thus presenting a promising strategy to remodel the TME toward an antitumor state and synergize with other immunotherapies against refractory tumors.<\/jats:p>","DOI":"10.1111\/cas.70489","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T11:33:49Z","timestamp":1785843229000},"page":"3121-3131","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeted Photo\u2010Induced Elimination of Tumor\u2010Associated Macrophages Suppresses Cancer Progression in a Murine Model"],"prefix":"10.1111","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-5363-2042","authenticated-orcid":false,"given":"Kohei","family":"Nakajima","sequence":"first","affiliation":[{"name":"Laboratory of Bioanalysis and Molecular Imaging, Graduate School of Pharmaceutical Sciences Hokkaido University  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomomi","family":"Uchiyama","sequence":"additional","affiliation":[{"name":"Laboratory of Bioanalysis and Molecular Imaging, Graduate School of Pharmaceutical Sciences Hokkaido University  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuki","family":"Funayama","sequence":"additional","affiliation":[{"name":"Laboratory of Bioanalysis and Molecular Imaging, Graduate School of Pharmaceutical Sciences Hokkaido University  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3898-2831","authenticated-orcid":false,"given":"Yuto","family":"Goto","sequence":"additional","affiliation":[{"name":"Laboratory of Bioanalysis and Molecular Imaging, Graduate School of Pharmaceutical Sciences Hokkaido University  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7742-7996","authenticated-orcid":false,"given":"Akari","family":"Mukaimine","sequence":"additional","affiliation":[{"name":"Institute for Chemical Reaction Design and Discovery (WPI\u2010ICReDD) Hokkaido University  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7810-9418","authenticated-orcid":false,"given":"Naoya","family":"Ieda","sequence":"additional","affiliation":[{"name":"Laboratory of Bioanalysis and Molecular Imaging, Graduate School of Pharmaceutical Sciences Hokkaido University  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3432-4519","authenticated-orcid":false,"given":"Mikako","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Laboratory of Bioanalysis and Molecular Imaging, Graduate School of Pharmaceutical Sciences Hokkaido University  Sapporo Hokkaido Japan"},{"name":"Institute for Chemical Reaction Design and Discovery (WPI\u2010ICReDD) Hokkaido University  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,4]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2019.06.001"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9010046"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-021-00484-9"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2022-329147"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40425-017-0257-y"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-13-3723"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2022.114884"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abd4550"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-02-266296"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-16-3261"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2554"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.9b00273"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.8b00565"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.13713"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.9b00547"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxad042"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-12-1298"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.14809"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajoms.2025.01.006"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2023.09.011"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-024-01040-6"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2020.101435"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2025.116152"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.37513"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15215117"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14030619"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2020.101926"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2021.121135"},{"issue":"9","key":"e_1_2_11_30_1","article-title":"CD146\u2010Targeted Multimodal Image\u2010Guided Photoimmunotherapy of Melanoma","volume":"6","author":"Wei W.","year":"2019","journal-title":"Advancement of Science"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.9b00738"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-15-1707"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2023.03.006"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.02.010"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2024.06.017"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-18-0546"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24087493"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-018-0014-x"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2023.02.016"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2022.2152248"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers17091584"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2016.01.027"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-013-9621-3"}],"container-title":["Cancer Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cas.70489","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/cas.70489","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/cas.70489","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T10:06:13Z","timestamp":1793613973000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/cas.70489"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,4]]},"references-count":42,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["10.1111\/cas.70489"],"URL":"https:\/\/doi.org\/10.1111\/cas.70489","archive":["Portico"],"relation":{},"ISSN":["1347-9032","1349-7006"],"issn-type":[{"value":"1347-9032","type":"print"},{"value":"1349-7006","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,4]]},"assertion":[{"value":"2025-08-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}