{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:53:27Z","timestamp":1791194007953,"version":"4.1.0"},"reference-count":70,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Science"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Biliary tract cancer (BTC) is a rare abdominal cancer with poor prognosis. Circulating tumor DNA (ctDNA) analysis with liquid biopsy offers a minimally invasive approach for cancer detection and disease monitoring. Given limited reports, this study aims to evaluate minimal residual disease (MRD) and longitudinal ctDNA monitoring in BTC patients. A total of 124 BTC patients were enrolled, with 111 evaluable for ctDNA analysis using a tumor\u2010informed approach. Baseline tumor tissue and plasma samples were collected for comprehensive genomic profiling (CGP). A customized ctDNA panel was developed based on CGP of resectable BTC tumors and public genomic databases for MRD assessment and longitudinal monitoring. ctDNA sequencing was performed using ultradeep targeted next\u2010generation sequencing. Associations between ctDNA detection and outcomes were analyzed. Preoperative ctDNA detection was associated with advanced pathological stage and significantly worse relapse\u2010free survival (RFS) and overall survival. Using this ctDNA panel, preoperative ctDNA was detected in 51.4% of cases, with TP53, KRAS, ARID1A, and SMAD4 being the most frequently mutated genes. During longitudinal monitoring, 57.8% of patients showed persistent ctDNA positivity or subsequent ctDNA detection before radiological recurrence. RFS was significantly shorter among patients with ctDNA positivity during longitudinal monitoring than among those with undetectable ctDNA (HR\u2009=\u20099.04; 95% CI, 3.56\u201323.97;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). Our customized panel detected BTC\u2010specific mutations in plasma using liquid biopsy, and ctDNA positivity was strongly associated with recurrence and survival outcomes. This panel has the potential to improve MRD assessment and longitudinal monitoring in BTC, enabling earlier intervention and improved patient stratification.\n                  <\/jats:p>","DOI":"10.1111\/cas.70552","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:13:12Z","timestamp":1791191592000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Assessment of\n                    <scp>MRD<\/scp>\n                    and Longitudinal Monitoring of Post\u2010Surgery Biliary Tract Cancer With a Customized\n                    <scp>ctDNA<\/scp>\n                    Panel"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3096-9344","authenticated-orcid":false,"given":"Yoshihito","family":"Shinohara","sequence":"first","affiliation":[{"name":"Department of Gastroenterological Surgery II Hokkaido University Faculty of Medicine  Sapporo Hokkaido Japan"},{"name":"Project for Development of Liquid Biopsy Diagnosis, Cancer Precision Medicine Center Japanese Foundation for Cancer Research  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toru","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery II Hokkaido University Faculty of Medicine  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasutoshi","family":"Kimura","sequence":"additional","affiliation":[{"name":"Department of Surgery, Surgical Oncology and Science Sapporo Medical University School of Medicine  Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Murakami","sequence":"additional","affiliation":[{"name":"Department of Surgery, Surgical Oncology and Science Sapporo Medical University School of Medicine  Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeyuki","family":"Kojima","sequence":"additional","affiliation":[{"name":"Division of Advanced Technology &amp; Development BML, Inc.  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Kawagoe Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanako C.","family":"Hatanaka","sequence":"additional","affiliation":[{"name":"Department of Surgical Pathology Hokkaido University Hospital  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5163-5511","authenticated-orcid":false,"given":"Ryo","family":"Okazaki","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery II Hokkaido University Faculty of Medicine  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yosuke","family":"Kitayama","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery II Hokkaido University Faculty of Medicine  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keita","family":"Fukuda","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery II Hokkaido University Faculty of Medicine  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio","family":"Pittella\u2010Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pathology of Cancer, Faculty of Health Sciences and Medicine University of Brasilia  Brasilia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Nakamura","sequence":"additional","affiliation":[{"name":"National Institutes of Biomedical Innovation, Health and Nutrition  Ibaraki Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Hirano","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery II Hokkaido University Faculty of Medicine  Sapporo Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2386-0698","authenticated-orcid":false,"given":"Siew\u2010Kee","family":"Low","sequence":"additional","affiliation":[{"name":"Project for Development of Liquid Biopsy Diagnosis, Cancer Precision Medicine Center Japanese Foundation for Cancer Research  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,5]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1038\/s41575-020-0310-z","article-title":"Cholangiocarcinoma 2020: The Next Horizon in Mechanisms and Management","volume":"17","author":"Banales J. 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