{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T17:22:11Z","timestamp":1778347331243,"version":"3.51.4"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,10,9]],"date-time":"2012-10-09T00:00:00Z","timestamp":1349740800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:p>Potent melanin production enhancement of human tyrosinase plasmid (pAH7\/Tyr, P) in mouse melanoma cells (B<jats:sub>16<\/jats:sub>F<jats:sub>10<\/jats:sub>) by Tat peptide (T) and an entrapment in elastic cationic niosomes (E) was described. The E composed of Tween 61\/cholesterol\/dodecyl dimethyl ammonium bromide at 1:1:0.5 molar ratio was prepared by freeze\u2010dried emptying liposomes method. PE at P\/E ratio of 1:160 w\/w and TPE at T\/P\/E ratio of 0.125:1:160, 0.25:1:160, and 0.5:1:160 w\/w\/w were prepared. The final concentration of the plasmid in the study was 4\u2003ng\/\u03bcL. By sulforhodamine B assay, PE and TPE complexes showed slight or no cytotoxic effect. The cells transfected with TPE (0.5:1:160) exhibited the highest enhancement of tyrosinase enzyme activity of 11.82\u2010, 7.67\u2010, 5.07\u2010, and 6.29\u2010folds of control, P, PE, and TP (0.5:1) and melanin production of 13.03\u2010, 8.46\u2010, 5.36\u2010, and 6.58\u2010folds of control, P, PE, and TP (0.5:1), respectively. The elastic cationic niosomes demonstrated an increase in thermal stability of P at 4\u2003\u00b1\u20032, 25\u2003\u00b1\u20032, and 45\u2003\u00b1\u20032\u2003\u00b0C. The vesicular size and the zeta potential values of PE and TPE complexes were slightly increased but still in the range of stable dispersion (out of \u00b130\u2003mV). These results indicated the high potential application of the TPE complexes for further investigation for vitiligo gene therapy.<\/jats:p>","DOI":"10.1111\/cbdd.12048","type":"journal-article","created":{"date-parts":[[2012,9,7]],"date-time":"2012-09-07T12:36:20Z","timestamp":1347021380000},"page":"953-960","source":"Crossref","is-referenced-by-count":6,"title":["Potent Melanin Production Enhancement of Human Tyrosinase Gene by Tat and an Entrapment in Elastic Cationic Niosomes: Potential Application in Vitiligo Gene Therapy"],"prefix":"10.1111","volume":"80","author":[{"given":"Jiradej","family":"Manosroi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Narinthorn","family":"Khositsuntiwong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Friedrich","family":"G\u00f6tz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolf G.","family":"Werner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Worapaka","family":"Manosroi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aranya","family":"Manosroi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,10,9]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/71889"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1359-6446(99)01433-6"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra032207"},{"key":"e_1_2_8_5_2","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1128\/jb.146.1.331-336.1981","article-title":"Mechanism of lysis of Escherichia coli by ethanol and other chaotropic agents","volume":"146","author":"Ingram L.O.","year":"1981","journal-title":"J Bacteriol"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.3109\/07388559709146617"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.03.127"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.34.945"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2011.01.020"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2011.04.003"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7827(02)00028-0"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-2494.2005.00264.x"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2008.04.033"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1208\/aapsj0901003"},{"key":"e_1_2_8_15_2","first-page":"747","article-title":"Peptide\u2010based delivery of proteins, nucleic acids and molecular probes into living cells","author":"Nitin N.","year":"2003","journal-title":"Summer Bioengineering Conference"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/js9800477"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12476630"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M200678200"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/jps.22104"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1166\/jbn.2011.1300"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2006.04.014"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.1166\/jbn.2011.1282"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2011.03.012"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc000056i"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.1080\/drd_10_4_251"},{"key":"e_1_2_8_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2007.05.045"}],"container-title":["Chemical Biology &amp; 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