{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T01:16:02Z","timestamp":1782522962000,"version":"3.54.5"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,12,26]],"date-time":"2013-12-26T00:00:00Z","timestamp":1388016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chem Biol Drug Des"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:p>Trastuzumab (<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>erceptin<jats:sup>\u00ae<\/jats:sup>) is a monoclonal antibody (m<jats:styled-content style=\"fixed-case\">Ab<\/jats:styled-content>) for specific ablation of <jats:styled-content style=\"fixed-case\">HER<\/jats:styled-content>2\u2010overexpressing malignant breast cancer cells. Intensification of antiproliferative activity of trastuzumab through construction of immunotoxins and nano\u2010immunoconjugates is a promising approach for treatment of cancer. In this study, trastuzumab was directly conjugated to diphtheria toxin (<jats:styled-content style=\"fixed-case\">DT<\/jats:styled-content>). Also, conjugates of trastuzumab and multiwalled carbon nanotubes (<jats:styled-content style=\"fixed-case\">MWCNT<\/jats:styled-content>) were constructed by covalent immobilization of trastuzumab onto <jats:styled-content style=\"fixed-case\">MWCNT<\/jats:styled-content>s. Then, antiproliferative activity of the fusion constructs against <jats:styled-content style=\"fixed-case\">HER<\/jats:styled-content>2\u2010overexpressing <jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">BR<\/jats:styled-content>\u20103 and also <jats:styled-content style=\"fixed-case\">HER<\/jats:styled-content>2\u2010negative <jats:styled-content style=\"fixed-case\">MCF<\/jats:styled-content>\u20107 cancer cell lines were examined. Cells treated with trastuzumab\u2010<jats:styled-content style=\"fixed-case\">MWCNT<\/jats:styled-content> conjugates were irradiated with near\u2010infrared (<jats:styled-content style=\"fixed-case\">NIR<\/jats:styled-content>) light. Efficient absorption of <jats:styled-content style=\"fixed-case\">NIR<\/jats:styled-content> radiation and its conversion to heat by <jats:styled-content style=\"fixed-case\">MWCNT<\/jats:styled-content>s can be resulted to thermal ablation of cancerous cells. Our results strongly showed that both trastuzumab\u2010<jats:styled-content style=\"fixed-case\">MWCNT<\/jats:styled-content> and trastuzumab\u2010<jats:styled-content style=\"fixed-case\">DT<\/jats:styled-content> conjugates were significantly efficient in the specific killing of <jats:styled-content style=\"fixed-case\">SK<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">BR<\/jats:styled-content>\u20103 cells. Targeting of <jats:styled-content style=\"fixed-case\">MWCNT<\/jats:styled-content>s to cancerous cells using trastuzumab followed by exposure of cells to <jats:styled-content style=\"fixed-case\">NIR<\/jats:styled-content> radiation was more efficient in repression of cell proliferation than treatment for cancer cells with trastuzumab\u2010<jats:styled-content style=\"fixed-case\">DT<\/jats:styled-content>. Our results also showed that conjugation linkers can significantly affect the cytotoxicity of <jats:styled-content style=\"fixed-case\">MWCNT<\/jats:styled-content>\u2010immunoconjugates. In conclusion, our data demonstrated that trastuzumab\u2010<jats:styled-content style=\"fixed-case\">MWCNT<\/jats:styled-content> is a promising nano\u2010immunoconjugate for killing of <jats:styled-content style=\"fixed-case\">HER<\/jats:styled-content>2\u2010overexpressing cancerous cells.<\/jats:p>","DOI":"10.1111\/cbdd.12244","type":"journal-article","created":{"date-parts":[[2013,10,12]],"date-time":"2013-10-12T16:26:45Z","timestamp":1381595205000},"page":"259-265","source":"Crossref","is-referenced-by-count":26,"title":["Ablation of Breast Cancer Cells Using Trastuzumab\u2010Functionalized Multi\u2010Walled Carbon Nanotubes and Trastuzumab\u2010Diphtheria Toxin Conjugate"],"prefix":"10.1111","volume":"83","author":[{"given":"Mojtaba","family":"Oraki Kohshour","sequence":"first","affiliation":[{"name":"Department of Immunology School of Medicine Ahvaz Jundishapur University of Medical Sciences Golestan Avenue, Ahvaz 15794\u201061357 Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sako","family":"Mirzaie","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Islamic Azad University Sanandaj Branch Pasdaran Avenue, Sanandaj 618 Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Majid","family":"Zeinali","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center Research Institute of Petroleum Industry (RIPI) West Blvd. Azadi Sports Complex, Tehran 14665\u2010137 Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mansour","family":"Amin","sequence":"additional","affiliation":[{"name":"Department of Microbiology School of Medicine Infectious and Tropical Diseases Research Center Ahvaz Jundishapur University of Medical Sciences Golestan Avenue, Ahvaz 618 Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Said Hakhamaneshi","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Kurdistan Cellular &amp; Molecular Research Center Kurdistan University of Medical Science Abidar Avenue, Sanandaj 6618634683 Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Jalili","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Kurdistan Cellular &amp; Molecular Research Center Kurdistan University of Medical Science Abidar Avenue, Sanandaj 6618634683 Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nader","family":"Mosaveri","sequence":"additional","affiliation":[{"name":"Razi Vaccine and Serum Research Institute Hessarak, Karaj 31975\/148 Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mostafa","family":"Jamalan","sequence":"additional","affiliation":[{"name":"Department of Microbiology School of Medicine Infectious and Tropical Diseases Research Center Ahvaz Jundishapur University of Medical Sciences Golestan Avenue, Ahvaz 618 Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,12,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000055396"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/1040-8428(94)00144-I"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1677\/erc.1.00600"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(01)00231-3"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1677\/erc.0.0080003"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(01)00230-1"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.2741\/grant"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4827(03)00002-8"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01392"},{"key":"e_1_2_8_11_1","first-page":"451","article-title":"Dynamic expression and activation of ERBB receptors in the developing mouse mammary gland","volume":"9","author":"Schroeder J.A.","year":"1998","journal-title":"Cell Growth Differ"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3798106"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1210477"},{"key":"e_1_2_8_14_1","first-page":"3","article-title":"Herceptin","volume":"18","author":"Shepard H.M.","year":"2008","journal-title":"Handb Exp Pharmacol"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1837"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.2012.685153"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/86684"},{"key":"e_1_2_8_18_1","first-page":"37","article-title":"Protein immobilization on carbon nanotubes via a two\u2010step process of diimide\u2010activated amidation","volume":"14","author":"Kuiyang J.","year":"2003","journal-title":"J Mater Chem"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5386.95"},{"key":"e_1_2_8_20_1","first-page":"767","article-title":"Ethylene glycol diglycidyl ether as a protein cross\u2010linker: a case study for cross\u2010linking of hemoglobin","volume":"81","author":"Xiuling L.","year":"2066","journal-title":"J Chem Technol Biotechnol"},{"key":"e_1_2_8_21_1","volume-title":"Bioconjugate Techniques","author":"Hermanson G.T.","year":"2008"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-9-351"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.23320"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1309\/5CWPU41AFR1VYM3F"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2005.04.017"},{"key":"e_1_2_8_26_1","first-page":"1490","article-title":"Approval summary: gemtuzumab ozogamicin in relapsed acute myeloid leukemia","volume":"7","author":"Bross P.F.","year":"2001","journal-title":"Clin Cancer Res"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2001.19.13.3244"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm9600398"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.092697199"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-01-0039"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/ar700108g"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1208\/aapsj080363"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(78)90099-5"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2008.06.017"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0803557105"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24659"}],"container-title":["Chemical Biology &amp; 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